Ring A
Biochim Biophys Acta. 1986 Apr 25;856(3):646-53. doi: 10.1016/0005-2736(86)90160-4.
Brief closures, so called flickers, gramicidin A channels were observed for glycerol monooleate/n-decane membranes for cesium chloride and hydrochloric acid solutions. The flickers, similar in nature to the flickers observed for physiological channels, were of the order of 1 ms and the interval between flickers was of the order of 50 ms. The flicker-duration and interval between flickers both decrease with voltage. The field dependence of the flickers is consistent with the hypothesis that the membrane forms a dimple when accomodating a dimer in the membrane and that the monomers, on breaking up, are associated over displacements of the order of 2 nm. For similar measurements for glycerol monoleate/hexadecane membranes only rare occurrences of flickers were observed. It is suggested that the flicker phenomenon is governed by the physical and chemical properties of the membrane and the influence of membrane thickness and interfacial free energy is emphasized.
在甘油单油酸酯/正癸烷膜中，对于氯化铯和盐酸溶液，观察到短暂关闭，即所谓的闪烁现象，这是由短杆菌肽A通道引起的。这些闪烁与在生理通道中观察到的闪烁本质相似，持续时间约为1毫秒，闪烁间隔约为50毫秒。闪烁持续时间和闪烁间隔都随电压降低。闪烁的场依赖性与以下假设一致：当膜容纳膜中的二聚体时会形成一个凹坑，并且单体在分解时会在约2纳米的位移上相互关联。对于甘油单油酸酯/十六烷膜的类似测量，仅观察到罕见的闪烁现象。有人认为，闪烁现象受膜的物理和化学性质支配，并强调了膜厚度和界面自由能的影响。
