绵羊聚合珠蛋白HpC的免疫化学特性
[Immunochemical characteristics of the ovine polymeric haptoglobin HpC].
作者信息
Beĭsembaeva R U, Abilova G M
出版信息
Biokhimiia. 1986 Feb;51(2):212-5.
It was found that haptoglobins of camel, cattle, horse, pig, rat, guinea pig and man form upon immunoelectrophoresis precipitation arcs with antibodies against polymeric sheep haptoglobin C, corresponding to alpha 2-globulins. The immunocrossreactivity of haptoglobins of man and various animal species towards antibody to sheep haptoglobin (100, 88.0, 75.2, 72.1, 56.3, 51.0, 41.3 and 28.0% for haptoglobin of sheep, camel, pig, cattle, man, rat, guinea pig and horse, respectively) was determined. The intensity of crossreactions between sheep haptoglobin and the proteins under study towards antibody to haptoglobin C reflects the similarity of their primary structure and, consequently, the immune homology of their molecules. Using quantitative titration, the antigenic valency values for human (6), sheep (5), cattle (4) and horse (3) haptoglobins were determined.
研究发现，骆驼、牛、马、猪、大鼠、豚鼠和人类的触珠蛋白在免疫电泳时与抗聚合羊触珠蛋白C的抗体形成沉淀弧，对应于α2球蛋白。测定了人类和各种动物物种的触珠蛋白对羊触珠蛋白抗体的免疫交叉反应性（羊、骆驼、猪、牛、人、大鼠、豚鼠和马的触珠蛋白分别为100%、88.0%、75.2%、72.1%、56.3%、51.0%、41.3%和28.0%）。羊触珠蛋白与所研究蛋白质之间针对触珠蛋白C抗体的交叉反应强度反映了它们一级结构的相似性，从而反映了它们分子的免疫同源性。通过定量滴定，测定了人（6）、羊（5）、牛（4）和马（3）触珠蛋白的抗原价数值。
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