Maddison S E
Dev Biol Stand. 1985;62:59-62.
A standardized microtest plate enzyme-linked immunosorbent assay (ELISA) was developed using the microsomal fraction of adult worms of Schistosoma mansoni (MAMA) as antigen. A standard reference serum pool was prepared and arbitrarily designated as having 100 activity units per microliter. The precision of the test, the suitability as a standard of the reference serum pool, and the J index as a method of determining the significant level of reactivity were evaluated. The predictability of the assay for patients with S. mansoni infections and for non-schistosomiasis patients was 95%. Preliminary studies with the truly quantitative microtest plate kinetic ELISA indicate that this assay is suitable for standardization for schistosomiasis and toxoplasmosis. The need for WHO International Reference Sera with designated International Units and for well-defined batteries of sera for evaluating the specificity and sensitivity of assays was emphasized.
利用曼氏血吸虫成虫微粒体部分（MAMA）作为抗原，开发了一种标准化的微量试验板酶联免疫吸附测定（ELISA）。制备了一个标准参考血清库，并任意指定其每微升具有100个活性单位。评估了该试验的精密度、参考血清库作为标准的适用性以及作为确定显著反应水平方法的J指数。该测定法对曼氏血吸虫感染患者和非血吸虫病患者的预测率为95%。对真正定量的微量试验板动力学ELISA的初步研究表明，该测定法适用于血吸虫病和弓形虫病的标准化。强调了对具有指定国际单位的世界卫生组织国际参考血清以及用于评估测定法特异性和敏感性的明确血清组的需求。
