Department of Biology, Southern Oregon State College, 97520, Ashland, OR, USA.
Planta. 1988 Dec;176(4):482-7. doi: 10.1007/BF00397654.
A polyclonal antiserum and monoclonal antibodies have been prepared to purified pollen exines of Calocedrus decurrens Florin. The location of the antigen is in the exine, as shown by light-and electron-microscopic immunocytochemistry. The greatest reduction in antibody binding follows treatment of the exine with chemicals known to alter sporopollenin. These results provide evidence that sporopollenin is antigenic. Exines of ten species of gymnosperms and angiosperms also bound the polyclonal antiserum, indicating similarity of sporopollenin structure.
已制备了针对柏科翠柏花粉外壁的多克隆抗血清和单克隆抗体。免疫细胞化学的光镜和电镜观察结果显示，该抗原位于外壁，且外壁经已知能改变花粉外壁物质的化学物质处理后，抗体结合量明显减少。这些结果表明花粉外壁物质是抗原。该多克隆抗血清还与十种裸子植物和被子植物的花粉外壁结合，表明花粉外壁物质结构具有相似性。
