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Immunofluorescent screening of monoclonal antibodies to surface antigens of animal and plant cells bound to polycarbonate membranes.

作者信息

Hough T, Singh M B, Smart I J, Knox R B

出版信息

J Immunol Methods. 1986 Aug 21;92(1):103-7. doi: 10.1016/0022-1759(86)90509-0.

DOI:10.1016/0022-1759(86)90509-0
PMID:3528295
Abstract

We have developed an immunofluorescent screening method in which animal or plant cells are immobilized without fixation on polycarbonate (Nuclepore) membranes. The technique does not involve centrifugation, and virtually no cells are lost during processing. Direct microscopic analysis of antibody binding provides an extremely sensitive and reproducible screening system for hybridoma supernatants.

摘要

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