在体外高频植物再生树豆（Tamarindus indica）。

In vitro high frequency plant regeneration of a tree legume Tamarindus indica (L.).

机构信息

Department of Bio-Sciences, M.D. University, 124001, Rohtak, India.

出版信息

Plant Cell Rep. 1991 Dec;10(11):569-73. doi: 10.1007/BF00232513.

Abstract

Optimal culture conditions for high frequency plant regeneration from excised cotyledons of Tamarindus indica were established. Maximum shoot bud differentiation (100%) occurred when the adaxial surface of the entire cotyledon (excised from 12-d old seedlings) was in contact with MS medium containing 5×10(-6)M BAP. On MS alone only roots were formed. Shoot or root formation was confined to nodal tissue at the top of the notch present on the adaxial surface at the proximal end of the cotyledon. Thirty-four to 95 shoots were regenerated in a 4 month period from individual cotyledons. Shoots were rooted on MS with 5.7×10(-6)M IAA. IAA (5.7×10(-7)M) alone induced complete plant formation. Regenerated plants were established in the soil with 70% success.

摘要

建立了从罗望子（Tamarindus indica）离体子叶中进行高频植物再生的最佳培养条件。当整个子叶（从 12 天大的幼苗上切下）的腹面与含有 5×10（-6）M BAP 的 MS 培养基接触时，芽的最大分化率（100%）发生。单独在 MS 上仅形成根。芽或根的形成仅限于子叶腹面近端缺口上的节间组织。从单个子叶在 4 个月内再生了 34 到 95 个芽。芽在含有 5.7×10（-6）M IAA 的 MS 上生根。单独的 IAA（5.7×10（-7）M）诱导了完整的植物形成。再生植物在土壤中的成活率为 70%。