Nayak N R, Patnaik S, Rath S P
Laboratory of Cytogenetics, Post-Graduate Department of Botany, Utkal University, Bhubaneswar, 751004, Orissa, India.
Plant Cell Rep. 1997 May;16(8):583-586. doi: 10.1007/BF01142328.
An efficient and reproducible procedure is described for the large-scale propagation of an epiphytic orchid,Acampe praemorsa (Roxb.) B latter and McCann using foliar explants. Shoot buds were induced in basal parts of foliar explants on Murashige and Skoog medium supplemented with N-benzyladenine (BA), kinetin (Kn) or thidiazuron (TDZ), the latter being most effective at 1.0 mg/1. Shoots formed to a TDZ-containing medium elongated following transfer to a substrate supplemented with 2.0 mg/l 1-naphthaleneacetic acid (NAA) and 0.5 mg/1 BA. NAA at lower concentrations had no beneficial effects on shoot regeneration, whether added to the medium along with BA, Kn or TDZ. However, it promoted shoot elongation and leaf expansion. Higher concentrations of NAA suppressed shoot regeneration. The frequency of shoot regeneration was greatly influenced by the developmental stage and orientation of the leaf. Shoots regenerated from the foliar explants were rooted successfully on MS medium containing 1.0 mg/l indole-3-butyric acid. The plantlets were acclimated and eventually transferred to a garden.
描述了一种高效且可重复的方法，用于使用叶片外植体大规模繁殖附生兰花尖囊兰（Acampe praemorsa (Roxb.) B latter and McCann）。在添加了N-苄基腺嘌呤（BA）、激动素（Kn）或噻二唑素（TDZ）的Murashige和Skoog培养基上，叶片外植体的基部诱导出芽，其中TDZ在1.0毫克/升时最有效。转移到添加有2.0毫克/升1-萘乙酸（NAA）和0.5毫克/升BA的培养基上后，在含TDZ的培养基上形成的芽会伸长。较低浓度的NAA对芽再生没有有益影响，无论它是与BA、Kn还是TDZ一起添加到培养基中。然而，它促进了芽的伸长和叶片扩展。较高浓度的NAA抑制芽再生。芽再生频率受叶片发育阶段和方向的极大影响。从叶片外植体再生的芽在含有1.0毫克/升吲哚-3-丁酸的MS培养基上成功生根。这些小植株经过驯化，最终转移到花园中。
