液体细胞分裂素脉冲诱导道格拉斯冷杉子叶不定芽的形成。

A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons.

机构信息

Department of Forest Science, Peavy Hall 154, Oregon State University, 97331-5705, Corvallis, Oregon, USA.

出版信息

Plant Cell Rep. 1991 Jun;10(3):156-60. doi: 10.1007/BF00232049.

DOI:10.1007/BF00232049

Abstract

The effects of high-concentration, 2-h liquid pulses of N(6)-benzylaminopurine (BA) and thidiazuron (TD) on adventitious bud and shoot formation were tested in cotyledons of Douglas-fir (Pseudotsuga menziesii). Seedling age proved important; on average, cotyledons from the youngest seedlings formed 10-fold more buds than cotyledons from the oldest seedlings. Optimal cytokinin concentrations for the youngest cotyledons were 400 and 800 μM BA, and 100 and 200 μM TD. Shoots developed best from buds induced with 300, 400, and 800 μM BA. Four gelling agents were tested; BRL agarose yielded more than three times the number of buds, and Gelrite nearly twice the number of buds, as either Sigma agar or Difco Bacto-Agar. One of the best treatments (400 μM BA, agarose) yielded more cotyledons with buds, and more buds per cotyledon, than when cytokinins were incorporated into the growth medium.

摘要

在花旗松（Pseudotsuga menziesii）子叶中测试了高浓度、2 小时液体脉冲的 N(6)-苄基氨基嘌呤（BA）和噻二唑隆（TD）对不定芽和芽形成的影响。苗龄证明很重要；平均而言，来自最年轻幼苗的子叶形成的芽是来自最老幼苗的子叶的 10 倍。最年轻子叶的最佳细胞分裂素浓度为 400 和 800 μM BA，以及 100 和 200 μM TD。用 300、400 和 800 μM BA 诱导的芽发育最好。测试了四种胶凝剂；BRL 琼脂糖产生的芽数是 Sigma 琼脂或 Difco Bacto-Agar 的三倍以上，Gelrite 产生的芽数是它们的两倍。最好的处理之一（400 μM BA，琼脂糖）产生的带芽子叶数量和每个子叶的芽数都多于将细胞分裂素掺入生长培养基时的数量。