Patel K R, Thorpe T A
Plant Physiology Research Group, Department of Biology, University of Calgary, Calgary, Alberta, Canada T2N 1N4.
Tree Physiol. 1986 Dec;1(3):289-301. doi: 10.1093/treephys/1.3.289.
A protocol has been developed for the in vitro production of plantlets of Engelmann spruce. Embryos and various parts of Engelmann spruce seedlings formed multiple shoots when cultured on defined media containing a cytokinin. The site and time of occurrence of the shoot buds, as well as their number, differed in the various explants. The frequency of shoot-forming explants was influenced by the salt formulation used, the type and concentrations of cytokinins and their mode of application. Development of buds was achieved by transferring the explants to basal medium containing no growth regulators. Elongation of shoots was stimulated by reducing the concentration of salts and sugar, addition of activated charcoal and transferral to increased photoperiod and lower temperature regimes. Maximum rooting was induced by giving a pulse of high concentration of indolebutyric acid to the shoots. The roots developed within 8-10 weeks and the regenerated plantlets were transferred to soil under non-sterile conditions.
已经开发出一种用于体外生产恩氏云杉植株的方案。当在含有细胞分裂素的特定培养基上培养时，恩氏云杉的胚胎和幼苗的各个部分形成了多个芽。不同外植体中芽的发生部位、时间以及数量各不相同。形成芽的外植体频率受所用盐配方、细胞分裂素的类型和浓度及其施用方式的影响。通过将外植体转移到不含生长调节剂的基础培养基上实现芽的发育。通过降低盐和糖的浓度、添加活性炭以及转移到增加的光周期和较低温度条件下，刺激芽的伸长。通过对芽施加高浓度吲哚丁酸脉冲诱导最大生根。根在8 - 10周内发育，再生的植株在非无菌条件下转移到土壤中。
