Crossin K L, Hoffman S, Grumet M, Thiery J P, Edelman G M
J Cell Biol. 1986 May;102(5):1917-30. doi: 10.1083/jcb.102.5.1917.
The sequential appearance of the extracellular matrix (ECM) protein, cytotactin, was examined during development of the chicken embryo by immunohistochemical techniques. Although cytotactin was identified as a molecule that mediates glia-neuron interactions, preliminary immunohistochemical localization of the molecule suggested that it was an ECM protein with a widespread but nonetheless more restricted distribution than either fibronectin or laminin. In the present study, it was found that cytotactin is first present in the gastrulating chicken embryo. It appears later in the basement membrane of the developing neural tube and notochord in a temporal sequence beginning in the cephalic regions and proceeding caudally. Between 2 and 3 d of development, the molecule is present at high levels in the early neural crest pathways (surrounding the neural tube and somites) but, in contrast to fibronectin and laminin, is not found in the lateral plate mesoderm or ectoderm. At later times, cytotactin is expressed extensively in the central nervous system, in lesser amounts in the peripheral nervous system, and in a number of nonneural sites, most prominently in all smooth muscles and in basement membranes of lung and kidney. Cytotactin appears in adult tissues with distributions that are similar to those seen in embryonic tissues. The findings raise the possibility that certain ECM proteins contribute to pattern formation in embryogenesis as a result of their restricted expression in a spatiotemporally regulated fashion at some sites but not at others.
运用免疫组织化学技术，对鸡胚发育过程中细胞外基质（ECM）蛋白——促触肌动蛋白的相继出现情况进行了检测。尽管促触肌动蛋白被鉴定为一种介导神经胶质-神经元相互作用的分子，但该分子初步的免疫组织化学定位表明，它是一种ECM蛋白，其分布广泛，但比纤连蛋白或层粘连蛋白的分布更具局限性。在本研究中，发现促触肌动蛋白最初出现在原肠胚形成期的鸡胚中。随后，它按时间顺序出现在发育中的神经管和脊索的基底膜中，从头部区域开始，向尾部延伸。在发育的第2至3天之间，该分子在早期神经嵴通路（围绕神经管和体节）中大量存在，但与纤连蛋白和层粘连蛋白不同的是，在侧板中胚层或外胚层中未发现。在随后的时期，促触肌动蛋白在中枢神经系统中广泛表达，在周围神经系统中表达量较少，并且在一些非神经部位表达，最显著的是在所有平滑肌以及肺和肾的基底膜中。促触肌动蛋白出现在成体组织中，其分布与在胚胎组织中所见的相似。这些发现增加了一种可能性，即某些ECM蛋白由于在某些部位以时空调节的方式受到限制表达，而在其他部位则不表达，从而对胚胎发生中的模式形成有贡献。
