用于¹³C代谢通量分析的体内核磁共振技术。

In vivo NMR for ¹³C Metabolic Flux Analysis.

作者信息

Roscher Albrecht, Troufflard Stéphanie, Taghki Abdelghani Idrissi

机构信息

Génie Enzymatique et Cellulaire, FRE CNRS 3580, Université de Picardie, Amiens Cedex, France.

出版信息

Methods Mol Biol. 2014;1090:143-52. doi: 10.1007/978-1-62703-688-7_10.

DOI:10.1007/978-1-62703-688-7_10

PMID:24222415

Abstract

The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed.

摘要

本文介绍了在植物代谢通量分析框架内体内核磁共振技术的应用。体内核磁共振技术能够可视化活跃的代谢网络，确定代谢和同位素稳态，并测量同位素稳态（静态）代谢通量分析不一定能获取的代谢通量。动力学数据可作为动态（非静态）代谢通量分析的输入。文中采用了一维和二维核磁共振方法。