Salon Christophe, Bataillé Marie-Paule, Gallardo Karine, Jeudy Christian, Santoni Anne-Lise, Trouverie Jacques, Voisin Anne-Sophie, Avice Jean-Christophe
INRA UMR 1347 Agroécologie INRA/Université de Bourgogne/AgroSup, Dijon Cedex, France.
Methods Mol Biol. 2014;1090:335-46. doi: 10.1007/978-1-62703-688-7_20.
In order to highlight our understanding on ecosystems functioning and resource sharing/competition, either in artificial environment or agrosystems, according to changes in the climatic conditions, it is necessary to measure accurately element fluxes within plants. Stable isotopes allow tracking safely and accurately on a short time frame the behavior of elements in plants. After a short review devoted to isotopic studies of elemental flux within plants, we explain how a direct multiple labelling study might be conducted in a plant, so as to measure over short time nitrogen and sulfur acquisition, and assimilates arising from a labelled source.
为了突出我们对人工环境或农业系统中生态系统功能以及资源共享/竞争随气候条件变化的理解，准确测量植物体内的元素通量很有必要。稳定同位素能够在短时间内安全、准确地追踪植物体内元素的行为。在简要回顾了关于植物体内元素通量的同位素研究后，我们解释了如何在植物中进行直接多重标记研究，以便在短时间内测量氮和硫的获取情况，以及来自标记源的同化产物。
