Manning Alyssa J, Peters Kimberly A, Peifer Mark, Rogers Stephen L
1Department of Biology, University of North Carolina at Chapel Hill, CB# 3280, Fordham Hall, South Road, Chapel Hill, NC 27599-3280, USA.
Sci Signal. 2013 Nov 12;6(301):ra98. doi: 10.1126/scisignal.2004427.
Epithelial morphogenesis is essential for shaping organs and tissues and for establishment of the three embryonic germ layers during gastrulation. Studies of gastrulation in Drosophila have provided insight into how epithelial morphogenesis is governed by developmental patterning mechanisms. We developed an assay to recapitulate morphogenetic shape changes in individual cultured cells and used RNA interference-based screening to identify Mist, a Drosophila G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptor (GPCR) that transduces signals from the secreted ligand Folded gastrulation (Fog) in cultured cells. Mist functioned in Fog-dependent embryonic morphogenesis, and the transcription factor Snail regulated expression of mist in zygotes. Our data revealed how a cell fate transcriptional program acts through a ligand-GPCR pair to stimulate epithelial morphogenetic shape changes.
上皮形态发生对于器官和组织的形成以及原肠胚形成过程中三个胚胎胚层的建立至关重要。对果蝇原肠胚形成的研究为上皮形态发生如何受发育模式机制调控提供了见解。我们开发了一种检测方法来重现单个培养细胞中的形态发生形状变化,并使用基于RNA干扰的筛选来鉴定Mist,一种果蝇G蛋白(异源三聚体鸟嘌呤核苷酸结合蛋白)偶联受体(GPCR),它在培养细胞中传导来自分泌配体折叠原肠胚(Fog)的信号。Mist在依赖Fog的胚胎形态发生中发挥作用,转录因子Snail调节合子中mist的表达。我们的数据揭示了细胞命运转录程序如何通过配体 - GPCR对来刺激上皮形态发生形状变化。