Department of General Surgery, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, China ; Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Affiliated Hospital of China Medical University, No. 155 Nanjing Bei Street, Heping District, Shenyang, Liaoning 110001, China.
Int J Endocrinol. 2013;2013:941568. doi: 10.1155/2013/941568. Epub 2013 Oct 10.
Estradiol (E2) promotes metastatic propensity. However, the detailed mechanism remains largely unknown. E-cadherin, vimentin, and MMP-9 play a dominant role in the metastatic process. We aimed to investigate the effects of E2 on metastatic potential of PTC cell line BCPAP and on E-cadherin, vimentin, and MMP-9 protein expression. PTC cell line BCPAP was evaluated for the presence of estrogen receptor (ER) by western blot analysis. The effects of E2, PPT (a potent ER α -selective agonist), and DPN (a potent ER β -selective agonist) on modulation of metastatic phenotype were determined by using in vitro scratch wound assay and invasion assay. In addition, the effects on E-cadherin, vimentin, and matrix metalloproteinase-9 (MMP-9) protein expression were evaluated by Western blot analysis. We found that BCPAP cells expressed ER α and ER β . E2 and PPT enhanced, but DPN inhibited, the migration and invasion of BCPAP cells in an in vitro experimental model system that is modulated by E-cadherin, vimentin, and MMP-9. These findings indicate that E2 induces the metastatic potential of BCPAP cells through ER α and ER β . The two ER subtypes play differential roles in modulation of BCPAP cell metastasis and the related molecule expressions including E-cadherin, vimentin, and MMP-9.
雌二醇（E2）促进转移倾向。然而，其详细机制在很大程度上仍然未知。E-钙黏蛋白、波形蛋白和 MMP-9 在转移过程中起主导作用。我们旨在研究 E2 对 PTC 细胞系 BCPAP 转移潜能的影响，以及对 E-钙黏蛋白、波形蛋白和 MMP-9 蛋白表达的影响。通过 Western blot 分析评估 PTC 细胞系 BCPAP 中是否存在雌激素受体（ER）。通过体外划痕实验和侵袭实验，确定 E2、PPT（一种有效的 ERα-选择性激动剂）和 DPN（一种有效的 ERβ-选择性激动剂）对转移表型的调节作用。此外，通过 Western blot 分析评估 E-钙黏蛋白、波形蛋白和基质金属蛋白酶-9（MMP-9）蛋白表达的影响。我们发现 BCPAP 细胞表达 ERα和 ERβ。E2 和 PPT 增强，但 DPN 抑制 BCPAP 细胞在体外实验模型系统中的迁移和侵袭，该系统受 E-钙黏蛋白、波形蛋白和 MMP-9 调节。这些发现表明，E2 通过 ERα 和 ERβ 诱导 BCPAP 细胞的转移潜能。两种 ER 亚型在调节 BCPAP 细胞转移和相关分子表达（包括 E-钙黏蛋白、波形蛋白和 MMP-9）方面发挥着不同的作用。
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