Katz D M, Black I B
J Neurosci. 1986 Apr;6(4):983-9. doi: 10.1523/JNEUROSCI.06-04-00983.1986.
Catecholaminergic (CA) phenotypic characteristics have recently been detected in adult sensory neurons, demonstrating that CA expression in the periphery extends beyond the sympathoadrenal axis. Consequently, we may now determine whether common principles underlie CA phenotypic organization in functionally and embryologically diverse populations of peripheral neurons. To begin defining sensory transmitter regulation, the present study examined the relationship of CA expression to sensory target innervation in cranial nerve ganglion cells of the adult rat. Retrograde labeling combined with tyrosine hydroxylase (TH) immunocytochemistry indicated that 80-90% of CA sensory neurons in the glossopharyngeal petrosal ganglion project peripherally in the carotid sinus nerve (CSN). Most of these cells innervate a single target, the carotid body, revealing a striking correlation between CA expression and the pattern of sensory target innervation. Furthermore, CSN transection resulted in a transient marked decrease in TH catalytic activity and immunoreactivity within 1 week. Activities returned to normal by 3 weeks. Thus, axotomy reversibly decreased sensory TH, reproducing effects observed with central CA neurons (Ross et al., 1975), but differing in certain aspects from observations with sympathetic CA neurons (Cheah and Geffen, 1973; Kessler and Black, 1979). To determine whether disruption of axonal transport itself decreased TH in petrosal neurons, colchicine cuffs were placed around the intact CSN. Colchicine blockade reproduced the effects of axotomy, suggesting that deranged transport, and not axonal damage per se, altered TH. Finally, we studied the role of sensory projections to the CNS by examining petrosal TH after glossopharyngeal nerve rhizotomy. In contrast to sequelae of peripheral axotomy, rhizotomy did not alter TH, suggesting that projections to the periphery predominate in regulation of sensory TH.(ABSTRACT TRUNCATED AT 250 WORDS)
最近在成年感觉神经元中检测到了儿茶酚胺能（CA）表型特征，这表明外周CA表达超出了交感肾上腺轴的范围。因此，我们现在可以确定在功能和胚胎学上不同的外周神经元群体中，CA表型组织是否存在共同的原则。为了开始确定感觉递质的调节，本研究检测了成年大鼠脑神经节细胞中CA表达与感觉靶神经支配的关系。逆行标记结合酪氨酸羟化酶（TH）免疫细胞化学表明，舌咽岩神经节中80-90%的CA感觉神经元通过颈动脉窦神经（CSN）向外周投射。这些细胞中的大多数支配单个靶标——颈动脉体，这揭示了CA表达与感觉靶神经支配模式之间的显著相关性。此外，CSN横断导致1周内TH催化活性和免疫反应性短暂显著降低。3周时活性恢复正常。因此，轴突切断可逆地降低了感觉TH，重现了在中枢CA神经元中观察到的效应（罗斯等人，1975年），但在某些方面与交感CA神经元的观察结果不同（谢和格芬，1973年；凯斯勒和布莱克，1979年）。为了确定轴突运输中断本身是否会降低岩神经节神经元中的TH，将秋水仙碱套环置于完整的CSN周围。秋水仙碱阻断重现了轴突切断的效应，表明运输紊乱而非轴突损伤本身改变了TH。最后，我们通过检查舌咽神经根切断术后岩神经节TH来研究感觉投射到中枢神经系统的作用。与外周轴突切断的后遗症相反，神经根切断并没有改变TH，这表明投射到外周在感觉TH的调节中占主导地位。（摘要截短至250字）
