Hwang C B, Greenspan J S, Shillitoe E J
J Oral Pathol. 1986 Mar;15(3):179-84. doi: 10.1111/j.1600-0714.1986.tb00602.x.
The distribution of 5 proteins of herpes simplex virus Type 1 was observed in cells that had been infected for various periods. The cells were stained with monoclonal antibodies to ICP4, ICP5, ICP6, ICP8, and gB, using the avidin-biotin-peroxidase complex (ABC) method. Each protein had a characteristic pattern of time of appearance and translocation by which it could be distinguished from the others. The sensitivity of the ABC technique, its ease of use, and the permanence of the preparations make this method well suited for the study of viral proteins.
观察了1型单纯疱疹病毒5种蛋白质在不同感染时间段的细胞中的分布情况。使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法，用针对ICP4、ICP5、ICP6、ICP8和gB的单克隆抗体对细胞进行染色。每种蛋白质都有其特有的出现时间和易位模式，借此可与其他蛋白质区分开来。ABC技术的敏感性、易用性以及制片的永久性使得该方法非常适合用于研究病毒蛋白。
