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抗生物素蛋白-生物素免疫测定法与三种市售免疫荧光试剂盒用于单纯疱疹病毒分型的比较。

Comparison of an avidin-biotin immunoassay with three commercially available immunofluorescence kits for typing of herpes simplex virus.

作者信息

Barnard D L, Johnson F B, Richards D F

出版信息

J Clin Pathol. 1985 Oct;38(10):1158-62. doi: 10.1136/jcp.38.10.1158.

DOI:10.1136/jcp.38.10.1158
PMID:2997305
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC499460/
Abstract

An avidin-biotin complex system was compared with three commercially available immunofluorescence kits for serotyping herpes simplex virus isolates from clinical specimens. Sensitivity values showed that the Electro-Nucleonics and Immulok reagents were useful in detecting the presence of virus, whereas the predictive values showed that the Syva and Immulok reagents possessed adequate discrimination between the herpes simplex virus serotypes. The avidin-biotin complex system was equal or superior to the immunofluorescence reagents tested both in detecting herpes simplex virus antigens in cell culture and in serotyping herpes simplex virus isolates.

摘要

将抗生物素蛋白-生物素复合物系统与三种市售免疫荧光试剂盒进行比较,用于对临床标本中的单纯疱疹病毒分离株进行血清分型。敏感性值表明,Electro-Nucleonics试剂和Immulok试剂可有效检测病毒的存在,而预测值表明,Syva试剂和Immulok试剂在单纯疱疹病毒血清型之间具有足够的区分能力。抗生物素蛋白-生物素复合物系统在检测细胞培养中的单纯疱疹病毒抗原以及对单纯疱疹病毒分离株进行血清分型方面,与所测试的免疫荧光试剂相当或更优。

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本文引用的文献

1
Utilization of the biotin/avidin system to amplify the sensitivity of the enzyme-linked immunosorbent assay (ELISA).利用生物素/抗生物素蛋白系统提高酶联免疫吸附测定(ELISA)的灵敏度。
J Immunol Methods. 1983 Feb 11;56(3):329-39. doi: 10.1016/s0022-1759(83)80022-2.
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Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex.利用生物素标记抗体和过氧化物酶生物素-抗生物素蛋白复合物检测细菌抗原的酶免疫测定法。
J Immunol Methods. 1983 Feb 11;56(3):319-27. doi: 10.1016/s0022-1759(83)80021-0.
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Biotin-avidin-amplified enzyme immunoassay for detection of herpes simplex virus antigen in clinical specimens.生物素-抗生物素蛋白放大酶免疫测定法检测临床标本中的单纯疱疹病毒抗原
J Clin Microbiol. 1983 Dec;18(6):1329-34. doi: 10.1128/jcm.18.6.1329-1334.1983.
4
Comparison of direct immunofluorescence and direct immunoperoxidase procedures for detection of herpes simplex virus antigen in lesion specimens.用于检测病变标本中单纯疱疹病毒抗原的直接免疫荧光法与直接免疫过氧化物酶法的比较。
J Clin Microbiol. 1983 Aug;18(2):445-8. doi: 10.1128/jcm.18.2.445-448.1983.
5
Typing of herpes simplex virus isolates by enzyme-linked immunosorbent assay: comparison between indirect and double-antibody sandwich techniques.通过酶联免疫吸附测定法对单纯疱疹病毒分离株进行分型:间接法与双抗体夹心技术的比较。
J Clin Microbiol. 1983 May;17(5):942-4. doi: 10.1128/jcm.17.5.942-944.1983.
6
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J Clin Microbiol. 1983 Mar;17(3):396-9. doi: 10.1128/jcm.17.3.396-399.1983.
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J Clin Microbiol. 1981 May;13(5):913-8. doi: 10.1128/jcm.13.5.913-918.1981.
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