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抗生物素蛋白-生物素免疫测定法与三种市售免疫荧光试剂盒用于单纯疱疹病毒分型的比较。

Comparison of an avidin-biotin immunoassay with three commercially available immunofluorescence kits for typing of herpes simplex virus.

作者信息

Barnard D L, Johnson F B, Richards D F

出版信息

J Clin Pathol. 1985 Oct;38(10):1158-62. doi: 10.1136/jcp.38.10.1158.

Abstract

An avidin-biotin complex system was compared with three commercially available immunofluorescence kits for serotyping herpes simplex virus isolates from clinical specimens. Sensitivity values showed that the Electro-Nucleonics and Immulok reagents were useful in detecting the presence of virus, whereas the predictive values showed that the Syva and Immulok reagents possessed adequate discrimination between the herpes simplex virus serotypes. The avidin-biotin complex system was equal or superior to the immunofluorescence reagents tested both in detecting herpes simplex virus antigens in cell culture and in serotyping herpes simplex virus isolates.

摘要

将抗生物素蛋白-生物素复合物系统与三种市售免疫荧光试剂盒进行比较,用于对临床标本中的单纯疱疹病毒分离株进行血清分型。敏感性值表明,Electro-Nucleonics试剂和Immulok试剂可有效检测病毒的存在,而预测值表明,Syva试剂和Immulok试剂在单纯疱疹病毒血清型之间具有足够的区分能力。抗生物素蛋白-生物素复合物系统在检测细胞培养中的单纯疱疹病毒抗原以及对单纯疱疹病毒分离株进行血清分型方面,与所测试的免疫荧光试剂相当或更优。

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