过氧化物酶体增殖物激活受体-α激动剂与拮抗剂对人脐静脉内皮细胞血管生成的比较
A comparison of peroxisome proliferator-activated receptor-α agonist and antagonist on human umbilical vein endothelial cells angiogenesis.
作者信息
Dana Nasim, Javanmard Shaghayegh H, Fazilati Mohammad, Pilehvarian Ali A
机构信息
Department of Physiology, Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
出版信息
Adv Biomed Res. 2013 Jul 30;2:54. doi: 10.4103/2277-9175.115792. eCollection 2013.
BACKGROUND
There are controversial reports about the antiangiogenic effects of peroxisome proliferator-activated receptor α (PPARα). In the current study, we compared the effects of PPARα agonist and antagonist on human umbilical vein endothelial cells (HUVECs) angiogenesis with matrigel assay.
MATERIALS AND METHODS
HUVECs (1 × 10(5) cells/well) treated with PPARα agonist (fenofibrate) and antagonist (GW6471) were cultured on matrigel for 24 h. Treated cells were stained with calcein and investigated by fluorescent microscopy. The obtained images were also analyzed by AngioQuant software. Finally, the data were analyzed using SPSS 15 software, Kruskal-Wallis and one way ANOVA.
RESULTS
Statistical analysis showed that fenofibrate significantly inhibit the tube formation (size, length, junction) (P < 0.05) but there was a trend to increased angiogenesis in GW6471 treated group (P > 0.05).
CONCLUSION
These results showed that PPARα agonist is effective in suppression of angiogenesis. Further studies are needed to confirm these results in in vivo studies.
背景
关于过氧化物酶体增殖物激活受体α（PPARα）的抗血管生成作用存在有争议的报道。在本研究中，我们通过基质胶试验比较了PPARα激动剂和拮抗剂对人脐静脉内皮细胞（HUVECs）血管生成的影响。
材料与方法
将用PPARα激动剂（非诺贝特）和拮抗剂（GW6471）处理的HUVECs（1×10⁵个细胞/孔）接种于基质胶上培养24小时。用钙黄绿素对处理后的细胞进行染色，并通过荧光显微镜观察。所得图像也用AngioQuant软件进行分析。最后，使用SPSS 15软件、Kruskal-Wallis检验和单因素方差分析对数据进行分析。
结果
统计分析表明，非诺贝特显著抑制管腔形成（大小、长度、连接）（P<0.05），但GW6471处理组有血管生成增加的趋势（P>0.05）。
结论
这些结果表明PPARα激动剂在抑制血管生成方面有效。需要进一步的研究在体内研究中证实这些结果。
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