Khazaei Majid, Moshayedi Muhammad Amin, Jervekani Massoud Teimouri, Aghili Shahrzad, Montazer Saeed, Dastjerdi Roshanak Mehdipour, Hashemzehi Fazlolah, Jazi Hourossadat Hashemi
Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2013 Jul 30;2:67. doi: 10.4103/2277-9175.115819. eCollection 2013.
This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.
EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.
Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.
Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.
本研究评估了L-精氨酸（一氧化氮（NO）前体）和L-NG-硝基精氨酸甲酯（L-NAME）（NO合酶抑制剂）对正常大鼠心肌毛细血管密度的影响。
18只雄性大鼠分为三组：第1组：接受L-NAME（10mg/kg/天；腹腔注射），第2组：接受L-精氨酸（50mg/kg/天；腹腔注射），第3组（对照组）接受生理盐水。3周后，采集血样并使用免疫组织化学方法评估心肌毛细血管密度。
对照组血清NO浓度为6.45±0.44μmol/升。与对照组相比，用L-精氨酸处理动物可增加血清NO浓度（分别为7.90±0.75与6.45±0.44μmol/升），而L-NAME则降低（分别为4.86±0.40与6.45±0.44μmol/升）。L-精氨酸显著增加血清血管内皮生长因子（VEGF）浓度（353.01±7.03与100.5±6.61pg/ml；P<0.05），然而，并未改变心肌毛细血管密度。
尽管L-精氨酸改变了一些血清血管生成因子，但L-精氨酸或L-NAME均不能改善正常大鼠的心肌毛细血管密度。
