雄激素受体共激活子 p120 亚型 β 在前列腺癌中高度表达。

Androgen receptor coactivator p120 subtype β is highly expressed in prostate cancer.

机构信息

Department of Urology, Gunma University Graduate School of Medicine, Maebashi, Japan.

出版信息

Prostate Int. 2013;1(1):10-5. doi: 10.12954/PI.12004. Epub 2013 Feb 3.

DOI:10.12954/PI.12004

PMID:24223396

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3821517/

Abstract

PURPOSE

The β form of p120 is reported to be a strong coactivator of the androgen receptor. We investigated the gene expression profiles of the α and β forms of p120 in prostate cancer cell lines, benign prostatic hyperplasia (BPH), nontreated prostate cancer (NTPC), and prostate cancer after androgen deprivation therapy (PCA-ADT).

METHODS

We obtained 154 prostate needle biopsy specimens (81 in BPH, 51 in NTPC, and 22 in PCA-ADT). Levels of p120α and β expression were determined by multiplex real-time polymerase chain reaction.

RESULTS

Prostate cancer cell lines, LNCaP, PC-3, DU-145, and LNCaP-LA, which is a derivative of LNCaP under androgen deprivation, expressed both p120α and p120β. p120α expression levels were significantly higher than those of p120β in all cell lines examined. In human prostate tissues, p120α expression was significantly higher than that of p120β in BPH and NTPC. p120α expression in BPH was significantly higher than in other groups. In contrast, p120β expression was significantly higher in NTPC and PCA-ADT than in BPH. Expression of the two forms of p120 was not correlated with age, prostate-specific antigen, or Gleason score.

CONCLUSIONS

The expression profiles of p120α and p120β significantly differ in cancerous and benign prostatic tissues.

摘要

目的

已有报道称 p120 的β 形式是雄激素受体的强共激活子。我们研究了 p120 的α 和β 形式在前列腺癌细胞系、良性前列腺增生（BPH）、未经治疗的前列腺癌（NTPC）和雄激素剥夺治疗后的前列腺癌（PCA-ADT）中的基因表达谱。

方法

我们获得了 154 例前列腺针吸活检标本（81 例 BPH、51 例 NTPC 和 22 例 PCA-ADT）。通过多重实时聚合酶链反应确定 p120α 和β 表达水平。

结果

前列腺癌细胞系 LNCaP、PC-3、DU-145 和 LNCaP-LA（雄激素剥夺下 LNCaP 的衍生物）均表达 p120α 和 p120β。在所有检测的细胞系中，p120α 的表达水平均显著高于 p120β。在人前列腺组织中，BPH 和 NTPC 中的 p120α 表达均显著高于 p120β。BPH 中的 p120α 表达明显高于其他组。相比之下，NTPC 和 PCA-ADT 中的 p120β 表达明显高于 BPH。两种形式的 p120 的表达与年龄、前列腺特异性抗原或 Gleason 评分无关。