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人B淋巴细胞中爱泼斯坦-巴尔病毒/C3d补体受体的纯化：纯化蛋白的抗原性和功能特性

Purification of the Epstein-Barr virus/C3d complement receptor of human B lymphocytes: antigenic and functional properties of the purified protein.

作者信息

Nemerow G R, Siaw M F, Cooper N R

出版信息

J Virol. 1986 May;58(2):709-12. doi: 10.1128/JVI.58.2.709-712.1986.

DOI:10.1128/JVI.58.2.709-712.1986
PMID:2422399
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC252969/
Abstract

The Epstein-Barr virus/C3d receptor (CR2) of human B lymphocytes was purified to homogeneity from Raji cells by immunoaffinity chromatography. The average yield of the 145-kilodalton receptor was 400 pmol (50 micrograms) per 10(10) cells, representing an approximate 75% recovery. The isolated 145-kilodalton protein was antigenically and functionally intact as it reacted with several anti-CR2 monoclonal antibodies and bound purified Epstein-Barr virus and C3d,g. These findings with the purified molecule provide an unequivocal demonstration of the dual receptor functions of this protein.

摘要

人B淋巴细胞的爱泼斯坦-巴尔病毒/C3d受体（CR2）通过免疫亲和层析从拉吉细胞中纯化至同质。每10¹⁰个细胞中145千道尔顿受体的平均产量为400皮摩尔（50微克），回收率约为75%。分离出的145千道尔顿蛋白质在抗原性和功能上均保持完整，因为它能与几种抗CR2单克隆抗体发生反应，并能结合纯化的爱泼斯坦-巴尔病毒和C3d,g。这些关于纯化分子的研究结果明确证明了该蛋白质的双重受体功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/85448339b343/jvirol00110-0485-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/163c245ed135/jvirol00110-0484-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/a75a0417f626/jvirol00110-0485-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/85448339b343/jvirol00110-0485-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/163c245ed135/jvirol00110-0484-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/a75a0417f626/jvirol00110-0485-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c69f/252969/85448339b343/jvirol00110-0485-b.jpg

相似文献

1
Purification of the Epstein-Barr virus/C3d complement receptor of human B lymphocytes: antigenic and functional properties of the purified protein.人B淋巴细胞中爱泼斯坦-巴尔病毒/C3d补体受体的纯化：纯化蛋白的抗原性和功能特性
J Virol. 1986 May;58(2):709-12. doi: 10.1128/JVI.58.2.709-712.1986.
2
Incorporation of the purified Epstein Barr virus/C3d receptor (CR2) into liposomes and demonstration of its dual ligand binding functions.将纯化的爱泼斯坦-巴尔病毒/C3d受体（CR2）整合到脂质体中，并证明其双配体结合功能。
J Immunol. 1986 Jun 1;136(11):4140-5.
3
Purification of the B lymphocyte receptor for the C3d fragment of complement and the Epstein-Barr virus by monoclonal antibody affinity chromatography, and assessment of its functional capacities.通过单克隆抗体亲和层析法纯化补体C3d片段和爱泼斯坦-巴尔病毒的B淋巴细胞受体，并评估其功能能力。
J Immunol Methods. 1986 Aug 21;92(1):79-87. doi: 10.1016/0022-1759(86)90506-5.
4
Biochemical and antigenic analysis of the Epstein Barr virus/C3d receptor (CR2).爱泼斯坦-巴尔病毒/C3d受体（CR2）的生化与抗原分析
J Immunol. 1986 Jun 1;136(11):4146-51.
5
Identification and characterization of the Epstein-Barr virus receptor on human B lymphocytes and its relationship to the C3d complement receptor (CR2).人B淋巴细胞上爱泼斯坦-巴尔病毒受体的鉴定与特性及其与C3d补体受体（CR2）的关系。
J Virol. 1985 Aug;55(2):347-51. doi: 10.1128/JVI.55.2.347-351.1985.
6
Identification of a spontaneously shed fragment of B cell complement receptor type two (CR2) containing the C3d-binding site.鉴定出一种自发脱落的含有C3d结合位点的二型B细胞补体受体（CR2）片段。
Complement. 1987;4(2):87-98. doi: 10.1159/000463012.
7
Structural requirements for C3d,g/Epstein-Barr virus receptor (CR2/CD21) ligand binding, internalization, and viral infection.C3d,g/爱泼斯坦-巴尔病毒受体（CR2/CD21）配体结合、内化及病毒感染的结构要求。
J Biol Chem. 1990 Jul 25;265(21):12293-9.
8
Epstein-Barr virus/complement fragment C3d receptor (CR2) reacts with p53, a cellular antioncogene-encoded membrane phosphoprotein: detection by polyclonal anti-idiotypic anti-CR2 antibodies.爱泼斯坦-巴尔病毒/补体片段C3d受体（CR2）与p53反应，p53是一种细胞抗癌基因编码的膜磷蛋白：通过多克隆抗独特型抗CR2抗体进行检测。
Proc Natl Acad Sci U S A. 1989 Dec;86(24):10054-8. doi: 10.1073/pnas.86.24.10054.
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Identification of gp350 as the viral glycoprotein mediating attachment of Epstein-Barr virus (EBV) to the EBV/C3d receptor of B cells: sequence homology of gp350 and C3 complement fragment C3d.鉴定gp350作为介导爱泼斯坦-巴尔病毒（EBV）与B细胞的EBV/C3d受体结合的病毒糖蛋白：gp350与C3补体片段C3d的序列同源性。
J Virol. 1987 May;61(5):1416-20. doi: 10.1128/JVI.61.5.1416-1420.1987.
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Epstein Barr virus/complement C3d receptor is an interferon alpha receptor.爱泼斯坦-巴尔病毒/补体C3d受体是一种α干扰素受体。
EMBO J. 1991 Apr;10(4):919-26. doi: 10.1002/j.1460-2075.1991.tb08025.x.

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Epstein-Barr virus binding to CD21 activates the initial viral promoter via NF-kappaB induction.爱泼斯坦-巴尔病毒与CD21结合通过诱导核因子κB激活初始病毒启动子。

本文引用的文献

1
Receptors for the complement C3d component and the Epstein-Barr virus are quantitatively coexpressed on a series of B-cell lines and their derived somatic cell hybrids.补体C3d成分的受体和爱泼斯坦-巴尔病毒的受体在一系列B细胞系及其衍生的体细胞杂种上定量共表达。
Cell Immunol. 1982 Sep 15;72(2):263-76. doi: 10.1016/0008-8749(82)90474-9.
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A one-step purification of membrane proteins using a high efficiency immunomatrix.使用高效免疫基质一步纯化膜蛋白。
J Biol Chem. 1982 Sep 25;257(18):10766-9.
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Isolation of Epstein Barr-virus and studies of its neutralization by human IgG and complement.
J Exp Med. 1997 Aug 29;186(5):731-7. doi: 10.1084/jem.186.5.731.
4
An EBV-transformed owl monkey B-lymphocyte cell line.一种爱泼斯坦-巴尔病毒转化的夜猴B淋巴细胞系。
In Vitro Cell Dev Biol Anim. 1997 Feb;33(2):88-91. doi: 10.1007/s11626-997-0028-z.
5
Column liquid chromatography of integral membrane proteins.整合膜蛋白的柱液相色谱法。
J Chromatogr. 1987 Jul 17;418:223-43. doi: 10.1016/0378-4347(87)80010-5.
6
Identification of gp350 as the viral glycoprotein mediating attachment of Epstein-Barr virus (EBV) to the EBV/C3d receptor of B cells: sequence homology of gp350 and C3 complement fragment C3d.鉴定gp350作为介导爱泼斯坦-巴尔病毒（EBV）与B细胞的EBV/C3d受体结合的病毒糖蛋白：gp350与C3补体片段C3d的序列同源性。
J Virol. 1987 May;61(5):1416-20. doi: 10.1128/JVI.61.5.1416-1420.1987.
7
Epstein-Barr virus regulates activation and processing of the third component of complement.爱泼斯坦-巴尔病毒调节补体第三成分的激活和加工。
J Exp Med. 1988 Sep 1;168(3):949-69. doi: 10.1084/jem.168.3.949.
8
Molecular cloning of the cDNA encoding the Epstein-Barr virus/C3d receptor (complement receptor type 2) of human B lymphocytes.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9194-8. doi: 10.1073/pnas.84.24.9194.
9
Epstein-Barr virus (EBV) induces expression of B-cell activation markers on in vitro infection of EBV-negative B-lymphoma cells.爱泼斯坦-巴尔病毒（EBV）在体外感染EBV阴性B淋巴瘤细胞时可诱导B细胞活化标志物的表达。
Proc Natl Acad Sci U S A. 1987 Nov;84(22):8060-4. doi: 10.1073/pnas.84.22.8060.
10
Soluble gp350/220 and deletion mutant glycoproteins block Epstein-Barr virus adsorption to lymphocytes.可溶性gp350/220和缺失突变糖蛋白可阻断爱泼斯坦-巴尔病毒吸附至淋巴细胞。
J Virol. 1988 Dec;62(12):4452-64. doi: 10.1128/JVI.62.12.4452-4464.1988.
爱泼斯坦-巴尔病毒的分离及其被人免疫球蛋白G和补体中和的研究。
J Immunol. 1981 Jul;127(1):272-8.
4
Analysis of C3-receptor activity on human B-lymphocytes and isolation of the complement receptor type 2 (CR2).人B淋巴细胞上C3受体活性分析及2型补体受体（CR2）的分离
Biochem J. 1984 Nov 15;224(1):75-86. doi: 10.1042/bj2240075.
5
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.聚丙烯酰胺凝胶中蛋白质的银染：一种具有增强均匀灵敏度的改良方法。
Anal Biochem. 1981 Nov 1;117(2):307-10. doi: 10.1016/0003-2697(81)90783-1.
6
Epstein-Barr virus receptor of human B lymphocytes is the C3d receptor CR2.人类B淋巴细胞的爱泼斯坦-巴尔病毒受体是C3d受体CR2。
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4510-4. doi: 10.1073/pnas.81.14.4510.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes.人类B淋巴细胞和T淋巴细胞的表面标志物。II. B淋巴细胞上爱泼斯坦-巴尔病毒受体的存在
J Exp Med. 1973 Dec 1;138(6):1365-78. doi: 10.1084/jem.138.6.1365.
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Neutrophils express a receptor for iC3b, C3dg, and C3d that is distinct from CR1, CR2, and CR3.中性粒细胞表达一种与CR1、CR2和CR3不同的iC3b、C3dg和C3d受体。
J Immunol. 1985 Apr;134(4):2571-9.
10
Binding of monoclonal antibody to the Epstein Barr virus (EBV)/CR2 receptor induces activation and differentiation of human B lymphocytes.单克隆抗体与爱泼斯坦-巴尔病毒（EBV）/CR2受体的结合可诱导人B淋巴细胞的激活和分化。
J Immunol. 1985 Nov;135(5):3068-73.