Harvey J W, Kaneko J J
Am J Vet Res. 1975 Oct;36(10):1511-3.
Glutathione reductase (GR) was studied in erythrocytes of horses, cats, dogs, and man. Glutathione reductase activity was measured in hemolysates with and without preincubation of hemolysates with flavinadenine dinucleotide. The percentage saturation of GR apoenzyme with cofactor (flavin-adenine dinucleotide) was lower in cats and dogs than in horses or man. The greatest amount of inactive apoenzyme was in feline erythrocytes. Total GR activity listed in order by species is cat greater than man greater than dog greater than horse. Kinetic constants for oxidized glutathione and reduced nicotinamide-adenine dinucleotide phosphate were determined in each species. Although kinetic constant (reduced nicotinamide-adenine dinucleotide phosphate) values for GR were similar, considerable species variation was observed in the kinetic constant (oxidized glutathione) for GR. The kinetic constant (oxidized glutathione) for equine GR was approximately 3 times that for human GR, with intermediate values determined for feline and canine GR.
对马、猫、狗和人类的红细胞中的谷胱甘肽还原酶（GR）进行了研究。在溶血产物预孵育黄素腺嘌呤二核苷酸和未预孵育的情况下，测定溶血产物中的谷胱甘肽还原酶活性。猫和狗中GR脱辅基酶与辅因子（黄素腺嘌呤二核苷酸）的饱和百分比低于马或人类。无活性脱辅基酶含量最高的是猫的红细胞。按物种列出的总GR活性顺序为：猫＞人类＞狗＞马。测定了每个物种中氧化型谷胱甘肽和还原型烟酰胺腺嘌呤二核苷酸磷酸的动力学常数。虽然GR的动力学常数（还原型烟酰胺腺嘌呤二核苷酸磷酸）值相似，但在GR的动力学常数（氧化型谷胱甘肽）方面观察到了相当大的物种差异。马GR的动力学常数（氧化型谷胱甘肽）约为人类GR的3倍，猫和狗GR的动力学常数处于中间值。
