• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

在小脑发育过程中，单个 climbing 纤维输入到浦肯野细胞的尖峰时间依赖性选择性增强。

Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development.

机构信息

1] Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan [2].

出版信息

Nat Commun. 2013;4:2732. doi: 10.1038/ncomms3732.

DOI:10.1038/ncomms3732
PMID:24225482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3868216/
Abstract

Shaping functional neural circuits in developing brain involves activity-dependent refinement of early-formed redundant synapses. In the developing cerebellum, a one-to-one connection between a climbing fibre (CF) and a Purkinje cell (PC) is established by selective strengthening of a single CF followed by elimination of surplus CFs. Here we investigate developmental changes in CF-mediated responses in PCs by using in vivo whole-cell recordings and two-photon Ca(2+) imaging. We show that each neonatal PC receives temporally clustered inputs from multiple CFs and temporal integration of these inputs is required to induce burst spiking and Ca(2+) rise in PCs. Importantly, a single CF input closest to PC's spike output is selectively strengthened during postnatal development. This spike timing-dependent selective strengthening is much less prominent in PC-selective P/Q-type voltage-dependent Ca(2+) channel knockout mice. Thus, spike timing- and Ca(2+)-dependent plasticity appears to underlie the selection of a single 'winner' CF and the establishment of mature CF-PC connections.

摘要

在发育中的大脑中形成功能性神经回路涉及早期形成的冗余突触的活动依赖性细化。在发育中的小脑，一个 climbing fibre (CF) 和一个 Purkinje 细胞 (PC) 之间的一一对应连接是通过选择性增强一个单一的 CF 并随后消除多余的 CF 来建立的。在这里，我们通过使用体内全细胞记录和双光子 Ca(2+)成像来研究 CF 介导的 PC 反应的发育变化。我们表明，每个新生的 PC 从多个 CF 接收时间上聚类的输入，并且这些输入的时间整合是诱导 PC 爆发性放电和 Ca(2+)升高所必需的。重要的是，在出生后发育过程中，离 PC 尖峰输出最近的单个 CF 输入被选择性地增强。在 PC 选择性 P/Q 型电压依赖性 Ca(2+)通道敲除小鼠中，这种尖峰时间依赖性选择性增强则不那么明显。因此，尖峰时间和 Ca(2+)依赖性可塑性似乎是选择单个“获胜”CF 和建立成熟 CF-PC 连接的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/fa3c56b15ac6/ncomms3732-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/2ebcee8c4bbf/ncomms3732-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/f88b1e4162b2/ncomms3732-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/bab52c79073d/ncomms3732-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/6a32d76c8ca7/ncomms3732-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/2239f5fcea77/ncomms3732-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/d434dbc0f2c8/ncomms3732-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/1533aaca4846/ncomms3732-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/fa3c56b15ac6/ncomms3732-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/2ebcee8c4bbf/ncomms3732-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/f88b1e4162b2/ncomms3732-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/bab52c79073d/ncomms3732-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/6a32d76c8ca7/ncomms3732-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/2239f5fcea77/ncomms3732-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/d434dbc0f2c8/ncomms3732-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/1533aaca4846/ncomms3732-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2460/3868216/fa3c56b15ac6/ncomms3732-f8.jpg

相似文献

1
Spike timing-dependent selective strengthening of single climbing fibre inputs to Purkinje cells during cerebellar development.在小脑发育过程中，单个 climbing 纤维输入到浦肯野细胞的尖峰时间依赖性选择性增强。
Nat Commun. 2013;4:2732. doi: 10.1038/ncomms3732.
2
Postsynaptic P/Q-type Ca2+ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum.浦肯野细胞中的突触后 P/Q 型钙通道介导了发育小脑的突触竞争和消除。
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9987-92. doi: 10.1073/pnas.1101488108. Epub 2011 May 31.
3
Maturation of Cerebellar Purkinje Cell Population Activity during Postnatal Refinement of Climbing Fiber Network.小脑浦肯野细胞群体活动在 climbing fiber 网络出生后细化期间的成熟。
Cell Rep. 2017 Nov 21;21(8):2066-2073. doi: 10.1016/j.celrep.2017.10.101.
4
Translocation of a "winner" climbing fiber to the Purkinje cell dendrite and subsequent elimination of "losers" from the soma in developing cerebellum.在发育中的小脑中，一条“获胜”的攀缘纤维向浦肯野细胞树突的移位以及随后从胞体中消除“失败”的纤维。
Neuron. 2009 Jul 16;63(1):106-18. doi: 10.1016/j.neuron.2009.06.008.
5
Activity-dependent maturation of climbing fiber to Purkinje cell synapses during postnatal cerebellar development.在小脑发育的产后阶段，与浦肯野细胞的连接的 climbing fiber 的活性依赖性成熟。
Cerebellum. 2012 Jun;11(2):449-50. doi: 10.1007/s12311-011-0337-3.
6
Roles of glutamate receptor delta 2 subunit (GluRdelta 2) and metabotropic glutamate receptor subtype 1 (mGluR1) in climbing fiber synapse elimination during postnatal cerebellar development.谷氨酸受体δ2亚基（GluRδ2）和代谢型谷氨酸受体1型（mGluR1）在出生后小脑发育过程中攀缘纤维突触消除中的作用。
J Neurosci. 2001 Dec 15;21(24):9701-12. doi: 10.1523/JNEUROSCI.21-24-09701.2001.
7
Calcium-dependent regulation of climbing fibre synapse elimination during postnatal cerebellar development.钙依赖性调节小脑发育过程中攀缘纤维突触消除。
J Physiol. 2013 Jul 1;591(13):3151-8. doi: 10.1113/jphysiol.2012.248252. Epub 2013 Jan 28.
8
Dendritic translocation establishes the winner in cerebellar climbing fiber synapse elimination.树突迁移确立了小脑 climbing fiber 突触消除中的优胜者。
J Neurosci. 2013 May 1;33(18):7641-53. doi: 10.1523/JNEUROSCI.4561-12.2013.
9
Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum.颗粒蛋白前体向 Sort1 的逆行信号传递拮抗发育小脑的突触消除。
Neuron. 2018 Feb 21;97(4):796-805.e5. doi: 10.1016/j.neuron.2018.01.018. Epub 2018 Feb 1.
10
GABAergic inhibition regulates developmental synapse elimination in the cerebellum.GABA 能抑制调节小脑发育性突触消除。
Neuron. 2012 Apr 26;74(2):384-96. doi: 10.1016/j.neuron.2012.02.032.

引用本文的文献

1
Molecular and Anatomical Strengthening of "Winner" Climbing Fiber Synapses in Developing Mouse Purkinje Cells.发育中小鼠浦肯野细胞中“获胜”攀爬纤维突触的分子与解剖学强化
J Neurosci. 2025 Apr 9;45(15):e2156242025. doi: 10.1523/JNEUROSCI.2156-24.2025.
2
Tbx1 haploinsufficiency leads to local skull deformity, paraflocculus and flocculus dysplasia, and motor-learning deficit in 22q11.2 deletion syndrome.Tbx1单倍体不足会导致22q11.2缺失综合征出现局部颅骨畸形、旁绒球和绒球发育异常以及运动学习缺陷。
Nat Commun. 2024 Dec 5;15(1):10510. doi: 10.1038/s41467-024-54837-3.
3
Nasal obstruction during development leads to defective synapse elimination, hypersynchrony, and impaired cerebellar function.

本文引用的文献

1
Synapse elimination in the developing cerebellum.发育小脑的突触消除。
Cell Mol Life Sci. 2013 Dec;70(24):4667-80. doi: 10.1007/s00018-013-1405-2. Epub 2013 Jun 28.
2
Calcium-dependent regulation of climbing fibre synapse elimination during postnatal cerebellar development.钙依赖性调节小脑发育过程中攀缘纤维突触消除。
J Physiol. 2013 Jul 1;591(13):3151-8. doi: 10.1113/jphysiol.2012.248252. Epub 2013 Jan 28.
3
The spike-timing dependence of plasticity.突触传递的时间依赖性可塑性。
发育过程中的鼻腔阻塞会导致突触消除缺陷、过度同步和小脑功能受损。
Commun Biol. 2024 Oct 23;7(1):1381. doi: 10.1038/s42003-024-07095-4.
4
Direct and indirect pathways for heterosynaptic interaction underlying developmental synapse elimination in the mouse cerebellum.在小鼠小脑发育性突触消除中，异突触相互作用的直接和间接途径。
Commun Biol. 2024 Jul 3;7(1):806. doi: 10.1038/s42003-024-06447-4.
5
Autistic-like behavior and cerebellar dysfunction in Bmal1 mutant mice ameliorated by mTORC1 inhibition.Bmal1 突变小鼠的类自闭症行为和小脑功能障碍可被 mTORC1 抑制所改善。
Mol Psychiatry. 2023 Sep;28(9):3727-3738. doi: 10.1038/s41380-022-01499-6. Epub 2022 Mar 17.
6
Mechanisms governing activity-dependent synaptic pruning in the developing mammalian CNS.调控哺乳动物中枢神经系统发育过程中活性依赖型突触修剪的机制。
Nat Rev Neurosci. 2021 Nov;22(11):657-673. doi: 10.1038/s41583-021-00507-y. Epub 2021 Sep 20.
7
Differential spatiotemporal development of Purkinje cell populations and cerebellum-dependent sensorimotor behaviors.浦肯野细胞群体和小脑依赖的感觉运动行为的差异时空发育。
Elife. 2021 May 11;10:e63668. doi: 10.7554/eLife.63668.
8
Distinct functional developments of surviving and eliminated presynaptic terminals.存活和消除的突触前终末的不同功能发展。
Proc Natl Acad Sci U S A. 2021 Mar 16;118(11). doi: 10.1073/pnas.2022423118.
9
Cerebellar Development and Circuit Maturation: A Common Framework for Spinocerebellar Ataxias.小脑发育与神经回路成熟：脊髓小脑共济失调的共同框架
Front Neurosci. 2020 Apr 2;14:293. doi: 10.3389/fnins.2020.00293. eCollection 2020.
10
Developmental Rewiring between Cerebellar Climbing Fibers and Purkinje Cells Begins with Positive Feedback Synapse Addition.小脑 climbing fibers 和浦肯野细胞之间的发育性重连始于正反馈突触的添加。
Cell Rep. 2019 Nov 26;29(9):2849-2861.e6. doi: 10.1016/j.celrep.2019.10.081.
Neuron. 2012 Aug 23;75(4):556-71. doi: 10.1016/j.neuron.2012.08.001.
4
GABAergic inhibition regulates developmental synapse elimination in the cerebellum.GABA 能抑制调节小脑发育性突触消除。
Neuron. 2012 Apr 26;74(2):384-96. doi: 10.1016/j.neuron.2012.02.032.
5
Climbing fiber synapse elimination in cerebellar Purkinje cells.小脑浦肯野细胞中的 climbing fiber 突触消除。
Eur J Neurosci. 2011 Nov;34(10):1697-710. doi: 10.1111/j.1460-9568.2011.07894.x.
6
Dendritic calcium signaling triggered by spontaneous and sensory-evoked climbing fiber input to cerebellar Purkinje cells in vivo.体内小脑浦肯野细胞被自发和感觉诱发性 climbing fiber 输入触发的树突钙信号。
J Neurosci. 2011 Jul 27;31(30):10847-58. doi: 10.1523/JNEUROSCI.2525-10.2011.
7
Postsynaptic P/Q-type Ca2+ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum.浦肯野细胞中的突触后 P/Q 型钙通道介导了发育小脑的突触竞争和消除。
Proc Natl Acad Sci U S A. 2011 Jun 14;108(24):9987-92. doi: 10.1073/pnas.1101488108. Epub 2011 May 31.
8
Development of axon-target specificity of ponto-cerebellar afferents.浦肯野纤维投射到小脑的轴突-靶标特异性的发育。
PLoS Biol. 2011 Feb 8;9(2):e1001013. doi: 10.1371/journal.pbio.1001013.
9
Translocation of a "winner" climbing fiber to the Purkinje cell dendrite and subsequent elimination of "losers" from the soma in developing cerebellum.在发育中的小脑中，一条“获胜”的攀缘纤维向浦肯野细胞树突的移位以及随后从胞体中消除“失败”的纤维。
Neuron. 2009 Jul 16;63(1):106-18. doi: 10.1016/j.neuron.2009.06.008.
10
Synapse elimination in the central nervous system.中枢神经系统中的突触消除。
Curr Opin Neurobiol. 2009 Apr;19(2):154-61. doi: 10.1016/j.conb.2009.05.002. Epub 2009 May 27.