Division of Fisheries Research, CSIRO Marine Laboratories, P.O. Box 120, 4163, Cleveland, Qld, Australia.
Microb Ecol. 1982 Jun;8(1):1-14. doi: 10.1007/BF02011456.
Bacteria in sediments from the surface aerobic layer (0-1 cm) and a deeper anaerobic layer (20-21 cm) of a seagrass bed were examined in section by transmission electron microscopy. Bacteria with a Gram-negative ultrastructure made up 90% of bacteria in the surface layer, and Gram-positive bacteria comprised 10%. In the anaerobic zone, Gram-negative bacteria comprised 70% and Gram-positive bacteria 30% of the bacterial population. These differences were highly significant and support predictions of these proportions made from muramic acid measurements and direct counting with fluorescence microscopy. Most cells were enveloped in extracellular slime layers or envelopes, some with considerable structural complexity. The trophic value to animals of these envelopes is discussed. A unique organism with spines was observed.
通过透射电子显微镜检查了海草床表面有氧层（0-1 厘米）和更深的厌氧层（20-21 厘米）沉积物中的细菌。革兰氏阴性超微结构的细菌占表层细菌的 90%，革兰氏阳性细菌占 10%。在厌氧区，革兰氏阴性细菌占细菌总数的 70%，革兰氏阳性细菌占 30%。这些差异非常显著，支持从 muramic 酸测量和荧光显微镜直接计数预测的这些比例。大多数细胞被细胞外粘液层或包膜包裹，有些具有相当复杂的结构。讨论了这些包膜对动物的营养价值。观察到一种具有刺的独特生物。
