大鼠脑中S-100b与胶质纤维酸性蛋白的免疫组织化学共定位

Immunohistochemical co-localization of S-100b and the glial fibrillary acidic protein in rat brain.

作者信息

Boyes B E, Kim S U, Lee V, Sung S C

出版信息

Neuroscience. 1986 Mar;17(3):857-65. doi: 10.1016/0306-4522(86)90050-3.

Abstract

A rabbit antiserum against purified bovine brain S-100b protein was produced and characterized by immunoassay and immunoblot analysis of electrophoretically resolved soluble brain proteins. Fluorescent immunohistochemistry was conducted in order to determine the cellular localization of the S-100b immunoreactivity. Double immunohistofluorescent experiments on adult rat brain tissue sections with the rabbit antiserum to S-100b and a rat monoclonal antibody to the glial fibrillary acidic protein resulted in immunolabelling of the same cells. This finding determines a strict astroglial localization of the S-100b immunoreactivity. In addition, the immunolabelling of astrocyte perikarya and processes by the S-100b immunohistochemistry is consistent with a cytoplasmic location of S-100b. In contrast, the glial fibrillary acidic protein immunohistochemistry predominantly labeled the fine fibrillary processes of the cells. The present report suggests that S-100b immunohistochemistry is of use for the specific identification and morphological description of astrocytes in the rat brain.

摘要

制备了一种针对纯化牛脑S - 100b蛋白的兔抗血清，并通过对电泳分离的可溶性脑蛋白进行免疫测定和免疫印迹分析对其进行表征。进行荧光免疫组织化学以确定S - 100b免疫反应性的细胞定位。用兔抗S - 100b血清和大鼠抗胶质纤维酸性蛋白单克隆抗体对成年大鼠脑组织切片进行双重免疫荧光实验，结果显示相同细胞被免疫标记。这一发现确定了S - 100b免疫反应性严格定位于星形胶质细胞。此外，S - 100b免疫组织化学对星形胶质细胞胞体和突起的免疫标记与S - 100b的细胞质定位一致。相比之下，胶质纤维酸性蛋白免疫组织化学主要标记细胞的细纤维状突起。本报告表明，S - 100b免疫组织化学可用于大鼠脑中星形胶质细胞的特异性鉴定和形态学描述。