Ridet J L, Alonso G, Chauvet N, Chapron J, Koenig J, Privat A
INSERM U. 336, Université Montpellier II, case 106, Place Eugène Bataillon, F-34095 Montpellier Cedex 05, France.
Cell Tissue Res. 1996 Jan;283(1):39-49. doi: 10.1007/s004410050510.
A specific monoclonal antiserum (Mab 6.17) inducing a strong immunostaining of the neuromuscular junction has been used to detect the possible occurrence of the corresponding antigen throughout the intact or lesioned central nervous system of adult rats. In intact animals, 6.17-immunolabeling was essentially detected in astrocyte-like structures located in white matter fasciculi of the brain, such as the optic tract, corpus callosum, fornix, and in the white matter of the spinal cord. The astroglial nature of such 6.17-immunolabeled profiles was verified by performing double or triple immunofluorescent labeling with Mab 6.17 and with specific antisera against astrocytic markers, such as S100 protein, glial fibrillary acidic protein and vimentin. In the white matter, all the structures reactive to Mab 6.17 were also reactive to antibodies against S100 protein, glial fibrillary acidic protein and vimentin. On the other hand, astrocytes of the grey matter that were immunoreactive to S100 and glial fibrillary acidic protein but negative to vimentin, were devoid of 6.17-immunoreactivity. After lesions including stab wound through the diencephalon or transection of the spinal cord, a marked increase of 6.17-immunostaining was noted in the regions surrounding the lesions. In these regions, 6.17-immunolabeling was associated with S100-, GFAP- and vimentin-positive astrocytes constituting the glial scar. The ultrastructural localization of 6.17-immunoreactivity indicated that, similar to glial fibrillary acidic protein and vimentin, the recognized antigen was mainly associated with gliofilaments. These observations indicate that, in the central nervous system of adult rats, Mab 6.17 recognizes a molecule associated with gliofilaments, which is essentially associated to reactive astrocytes expressing high levels of vimentin.
一种能在神经肌肉接头处引发强烈免疫染色的特异性单克隆抗血清（Mab 6.17）已被用于检测成年大鼠完整或受损中枢神经系统中相应抗原的可能存在情况。在完整动物中，6.17免疫标记主要在位于脑白质束（如视束、胼胝体、穹窿）以及脊髓白质中的星形胶质样结构中检测到。通过用Mab 6.17以及针对星形胶质细胞标志物（如S100蛋白、胶质纤维酸性蛋白和波形蛋白）的特异性抗血清进行双重或三重免疫荧光标记，证实了这种6.17免疫标记的结构具有星形胶质细胞的性质。在白质中，所有对Mab 6.17有反应的结构也对针对S100蛋白、胶质纤维酸性蛋白和波形蛋白的抗体有反应。另一方面，对S100和胶质纤维酸性蛋白有免疫反应但对波形蛋白呈阴性的灰质星形胶质细胞没有6.17免疫反应性。在包括通过间脑刺伤或脊髓横断在内的损伤后，在损伤周围区域观察到6.17免疫染色显著增加。在这些区域，6.17免疫标记与构成胶质瘢痕的S100、GFAP和波形蛋白阳性星形胶质细胞相关。6.17免疫反应性的超微结构定位表明，与胶质纤维酸性蛋白和波形蛋白类似，所识别的抗原主要与胶质丝相关。这些观察结果表明，在成年大鼠的中枢神经系统中，Mab 6.17识别一种与胶质丝相关的分子，该分子主要与表达高水平波形蛋白的反应性星形胶质细胞相关。
