Wientzek Angelika, Floegel Anna, Knüppel Sven, Vigl Matthaeus, Drogan Dagmar, Adamski Jerzy, Pischon Tobias, Boeing Heiner
Dept. of Epidemiology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany.
Int J Sport Nutr Exerc Metab. 2014 Apr;24(2):215-26. doi: 10.1123/ijsnem.2013-0048. Epub 2013 Nov 13.
The aim of our study was to investigate the relationship between objectively measured physical activity (PA) and cardiorespiratory fitness (CRF) and serum metabolites measured by targeted metabolomics in a population- based study. A total of 100 subjects provided 2 fasting blood samples and engaged in a CRF and PA measurement at 2 visits 4 months apart. CRF was estimated from a step test, whereas physical activity energy expenditure (PAEE), time spent sedentary and time spend in vigorous activity were measured by a combined heart rate and movement sensor for a total of 8 days. Serum metabolite concentrations were determined by flow injection analysis tandem mass spectrometry (FIA-MS/MS). Linear mixed models were applied with multivariable adjustment and p-values were corrected for multiple testing. Furthermore, we explored the associations between CRF, PA and two metabolite factors that have previously been linked to risk of Type 2 diabetes. CRF was associated with two phosphatidylcholine clusters independently of all other exposures. Lysophosphatidylcholine C14:0 and methionine were significantly negatively associated with PAEE and sedentary time. CRF was positively associated with the Type 2 diabetes protective factor. Vigorous activity was positively associated with the Type 2 diabetes risk factor in the mutually adjusted model. Our results suggest that CRF and PA are associated with serum metabolites, especially CRF with phosphatidylcholines and with the Type 2 diabetes protective factor. PAEE and sedentary time were associated with methionine. The identified metabolites could be potential mediators of the protective effects of CRF and PA on chronic disease risk.
在一项基于人群的研究中，我们的研究目的是调查客观测量的身体活动（PA）与心肺适能（CRF）以及通过靶向代谢组学测量的血清代谢物之间的关系。共有100名受试者提供了2份空腹血样，并在相隔4个月的2次访视时进行了CRF和PA测量。CRF通过台阶试验估算，而身体活动能量消耗（PAEE）、久坐时间和剧烈活动时间则通过心率和运动传感器组合测量，共测量8天。血清代谢物浓度通过流动注射分析串联质谱（FIA-MS/MS）测定。应用线性混合模型进行多变量调整，并对p值进行多重检验校正。此外，我们探讨了CRF、PA与先前与2型糖尿病风险相关的两个代谢物因子之间的关联。CRF与两个磷脂酰胆碱簇相关，独立于所有其他暴露因素。溶血磷脂酰胆碱C14:0和蛋氨酸与PAEE和久坐时间显著负相关。CRF与2型糖尿病保护因子呈正相关。在相互调整模型中，剧烈活动与2型糖尿病风险因子呈正相关。我们的结果表明，CRF和PA与血清代谢物相关，尤其是CRF与磷脂酰胆碱以及2型糖尿病保护因子相关。PAEE和久坐时间与蛋氨酸相关。所鉴定的代谢物可能是CRF和PA对慢性病风险保护作用的潜在介质。
