氯化钙是烟草原生质体中抗蚜的主要成分。

Calcium chloride as a major component contributing to aphid resistance ofNicotiana benthamiana.

机构信息

Leaf Tobacco Research Laboratory, Japan Tobacco Inc., Idei 1900, Oyama, 323, Tochigi, Japan.

出版信息

J Chem Ecol. 1996 Sep;22(9):1579-89. doi: 10.1007/BF02272399.

Abstract

Substances with antiaphid activity were extracted from the leaf surface of aphid-resistantN. benthamiana and purified. Sugar esters and diacyl glycerols were isolated from the chloroform extract, but only diacyl glycerols showed significant activity. However, the major activity was found in the water extract rather than in the chloroform extract. From the water extract calcium chloride was isolated as the most abundant active substance. Only calcium chloride showed significant activity among several calcium salts and chlorides of several metals that are abundant in plants. Calcium contents per unit area of leaf surface ofN. benthamiana and aphid-resistantN. gossei were almost equal to each other and 10-100 times higher than that of aphid-susceptibleN. tabacum.

摘要

从抗蚜烟草原生质体叶片表面提取具有抗蚜活性的物质并进行纯化。从氯仿提取物中分离出糖酯和二酰基甘油，但只有二酰基甘油表现出显著的活性。然而，主要活性物质存在于水提取物中，而不是氯仿提取物中。从水提取物中分离出氯化钙作为最丰富的活性物质。在几种植物中含量丰富的几种钙盐和金属氯化物中，只有氯化钙表现出显著的活性。烟草原生质体和抗蚜烟草原生质体单位叶面积的钙含量彼此几乎相等，是烟蚜易感品种的 10-100 倍。