Research Institute for Photosynthesis, University of Sheffield, S10 2TN, Sheffield, UK.
Planta. 1987 May;171(1):65-72. doi: 10.1007/BF00395068.
Using a photoacoustic technique it has been possible to observe fast oxygen evolution and uptake transients at a high time resolution (approx. 0.2 s), when a dark-adapted leaf is reilluminated. There is initially a rapid pulse of oxygen evolution, correlated with the initial fluorescence rise (total duration under the experimental conditions used about 1-2 s), corresponding presumably to the photoreduction of the plastoquinone pool. This phenomenon may be utilized to calibrate the oxygen-evolution photoacoustic signal. The first pulse is followed by a series of slower bursts of oxygen uptake and evolution, reflecting various pools which are expressed following sequential activation of various parts of the photosynthetic apparatus, until achievement of a steady state.
利用光声技术，可以在高时间分辨率（约 0.2 秒）下观察到暗适应叶片重新被照射时的快速氧气产生和吸收瞬变。最初会有一个快速的氧气产生脉冲，与初始荧光上升相关（在使用的实验条件下总持续时间约为 1-2 秒），推测对应于质体醌库的光还原。这种现象可用于校准氧气产生光声信号。第一个脉冲之后是一系列较慢的氧气吸收和释放爆发，反映了各种池，这些池在光合作用装置的各个部分依次激活后表现出来，直到达到稳定状态。
