Department of Fisheries, Faculty of Agriculture, Kyoto University, Kyoto, Japan.
Microb Ecol. 1981 Jun;7(2):123-30. doi: 10.1007/BF02032494.
Eighteen strains of obligately oligotrophic bacteria that grow in a medium containing 1 mg of organic carbon per liter and do not grow in a rich medium (5 g/liter of nutrient) were isolated as dominant organisms from the oligotrophic water of Lake Biwa. The growth properties of these, especially of five strains, were examined. The maximum cell yield ranged from 8.5×10(4)/ml to 2.3×10(6)/ml, and their doubling times ranged from 6.6/h to 11.8/h in LT10(-4) medium (0.5 mg trypticase and 0.05 mg yeast extract in 1 liter of filtered and aged lake water). They also showed good growth in lake water medium without adding nutrients. The optimum concentrations for their growth were 5 mg/1, 5-50 mg/1, 50 mg/1, or 500 mg/1, depending on the strains. They utilized glutamate, glycine, serine, and glycolate, but not acetate, proline, or leucine. Several properties were examined. Their growth properties were very different from those of oligotrophs or oligocarbophiles isolated by other researchers.
从琵琶湖贫营养水中分离到 18 株专性寡营养细菌，它们在含有 1mg/L 有机碳的培养基中生长，而在富营养培养基（5g/L 营养物）中不生长。这些菌的生长特性，尤其是 5 株菌的生长特性，得到了研究。最大细胞产量范围为 8.5×10(4)/ml 至 2.3×10(6)/ml，在 LT10(-4)培养基（每升过滤老化湖水中含有 0.5mg 胰蛋白酶和 0.05mg 酵母提取物）中的倍增时间范围为 6.6/h 至 11.8/h。它们在不添加营养物的湖水培养基中也能良好生长。根据菌株的不同，其生长的最佳浓度为 5mg/1、5-50mg/1、50mg/1 或 500mg/1。它们利用谷氨酸、甘氨酸、丝氨酸和乙二酸盐，但不利用乙酸盐、脯氨酸或亮氨酸。对其进行了多项性质检测，它们的生长特性与其他研究者分离的寡营养菌或寡养碳细菌的生长特性有很大不同。
