Akagi Y, Taga N
Can J Microbiol. 1980 Apr;26(4):454-9. doi: 10.1139/m80-075.
The transport systems of the oligotrophic bacterium 486 for D-glucose and L-proline have been compared with those of the heterotrophic bacterium RP-303. Kinetic studies demonstrated that the rates of D-glucose and L-proline uptake by the two organisms were saturable processes. The apparent Km values of strain 486 for D-glucose and L-proline were 13.0 microM and 0.2 microM, respectively, whereas those of strain RP-303 were 3.2 microM for D-glucose and 1.8 microM for L-proline. Competition studies indicated that the D-glucose transport system of each bacterium was highly specific for D-glucose. The L-proline transport system of the oligotrophic bacterium 486 had a broad specificity, whereas that of the heterotrophic bacterium RP-303 had a narrow one.
已将贫营养细菌486的D-葡萄糖和L-脯氨酸转运系统与异养细菌RP-303的转运系统进行了比较。动力学研究表明，两种生物体对D-葡萄糖和L-脯氨酸的摄取速率是可饱和过程。菌株486对D-葡萄糖和L-脯氨酸的表观Km值分别为13.0微摩尔和0.2微摩尔，而菌株RP-303对D-葡萄糖的表观Km值为3.2微摩尔，对L-脯氨酸的表观Km值为1.8微摩尔。竞争研究表明，每种细菌的D-葡萄糖转运系统对D-葡萄糖具有高度特异性。贫营养细菌486的L-脯氨酸转运系统具有广泛的特异性，而异养细菌RP-303的L-脯氨酸转运系统具有较窄的特异性。
