John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK.
Planta. 1987 Jul;171(3):302-12. doi: 10.1007/BF00398675.
A series of monoclonal antibodies and a polyclonal antiserum have been used to investigate the localisation and pathway of biosynthesis of the cell-wall hydroxyproline-rich glycoprotein 2BII in the alga Chlamydomonas reinhardii. Glyco-protein precursors were detected within the endoplasmic reticulum using a polyclonal antiserum raised to the deglycosylated 2BII. Monoclonal antibodies which are known to recognise different carbohydrate epitopes of 2BII were found to label two distinct regions of the Golgi stack. The immunolabelling results demonstrate that there is compartmentation of protein synthesis and glycosylation steps for these O-glycosidically linked glycoproteins. Newly synthesised glycoproteins are transported from the Golgi apparatus to the cell surface via two distinct routes. They then undergo assembly into a cell wall, the inner wall layer being formed first and probably functionaing as a template within which the outer crystalline wall layers are assembled.
已使用一系列单克隆抗体和多克隆抗血清来研究藻类莱茵衣藻细胞壁羟脯氨酸丰富的糖蛋白 2BII 的定位和生物合成途径。使用针对去糖基化 2BII 的多克隆抗血清检测到内质网内的糖蛋白前体。发现已知识别 2BII 不同碳水化合物表位的单克隆抗体标记着高尔基体堆叠的两个不同区域。免疫标记结果表明，这些 O-糖基化糖蛋白的蛋白质合成和糖基化步骤存在区室化。新合成的糖蛋白通过两条不同的途径从高尔基体运送到细胞表面。然后它们组装成细胞壁，内层首先形成，并可能作为组装外层结晶层的模板。
