John Innes Institute, Colney Lane, NR4 7UH, Norwich, U.K..
Planta. 1976 Jan;131(2):165-71. doi: 10.1007/BF00389990.
A soluble extract from purified cell walls of C. reinhardii has been separated by gel filtration into three fractions which together account for 94% of the cell wall. The major fraction (accounting for 70% of the extract) is a glycoprotein, with a molecular wt. in sodium perchlorate of 298,000, which can be split into 4 electrophoretically distinct species. It contains 35% protein with high levels of hydroxyproline, arabinose and galactose, and is capable of self assembly into crystalline structures identical to those found within the cell wall. The second fraction (25% of the extract) is a similar glycoprotein, but contains 24% protein, a higher proportion of mannose, and is incapable of self assembly. The third fraction (3-6% of the extract) is shown to be an adsorbed impurity from the growth medium used.
从纯化的 C. reinhardii 细胞壁中提取的可溶性提取物已通过凝胶过滤分离成三个部分，这些部分共同占细胞壁的 94%。主要部分（占提取物的 70%）是一种糖蛋白，在高氯酸钠中的分子量为 298000，可以分裂成 4 种电泳明显不同的物种。它含有 35%的蛋白质，具有高水平的羟脯氨酸、阿拉伯糖和半乳糖，并且能够自我组装成与细胞壁内相同的结晶结构。第二部分（提取物的 25%）是一种类似的糖蛋白，但含有 24%的蛋白质、更高比例的甘露糖，并且不能自我组装。第三部分（提取物的 3-6%）被证明是来自所用生长培养基的吸附杂质。
