Department of Chemistry, University of Illinois, 600 S. Mathews St., 61801, Urbana, Illinois.
J Chem Ecol. 1996 Mar;22(3):513-39. doi: 10.1007/BF02033653.
Hexane extracts of leaves of 307 accessions from 73 host plant species ofHelicoverpa zea were analyzed by gas chromatography (GC) and used forH. zea oviposition and neonate larvae orientation bioassays. The gas chromatographic (GC) retention times of compounds statistically associated with behavioral activity were identified by correlation of GC peak area with oviposition and larval orientation preferences. Although taxonomically diverse in their origin, compounds for study were purified from extracts of species of the genusLycopersicon, due to their relative abundance. The structures of eight long-chain alkanes associated with oviposition preference were assigned by mass spectrometry, and the structures of five similarly associated organic acids and a terpenoid alkene were identified by(1)H and(13)C nuclear magnetic resonance spectroscopy. The structures of a number of other phytochemicals from the plant leaves were identified for comparative purposes, including a previously unknown terpene, 7-epizingiberene. Bioassays were performed on the isolated acids and on the alkane wax fractions of severalLycopersicon species, and significant differences were found in oviposition stimulation for both classes of compounds. Of the hundreds of compounds found in the extracts, none were observed to act as oviposition deterrents. The results of these bioassays may be useful in explaining the broad host range ofH. zea, as well as the process and evolution of host plant selection for oviposition.
从 73 种茄夜蛾（Helicoverpa zea）宿主植物的 307 个个体中提取的叶片正己烷提取物通过气相色谱（GC）进行分析，并用于茄夜蛾产卵和幼体幼虫取向生物测定。通过将 GC 峰面积与产卵和幼虫取向偏好相关联，确定与行为活性统计学相关的化合物的气相色谱（GC）保留时间。尽管在起源上具有不同的分类地位，但由于相对丰度，研究用化合物是从番茄属（Lycopersicon）的物种提取物中纯化的。与产卵偏好相关的 8 种长链烷烃的结构通过质谱法分配，与 5 种类似相关的有机酸和萜烯烯的结构通过（1）H 和（13）C 核磁共振波谱法确定。为了比较的目的，还鉴定了来自植物叶片的许多其他植物化学物质的结构，包括一种先前未知的萜烯，7-表姜烯。对几种番茄属物种的分离酸和烷烃蜡馏分进行了生物测定，发现这两类化合物在产卵刺激方面存在显著差异。在提取物中发现的数百种化合物中，没有一种被观察到具有产卵驱避作用。这些生物测定的结果可能有助于解释茄夜蛾广泛的宿主范围，以及宿主植物产卵选择的过程和进化。
