Department of Surgical and Morphological Sciences, Section of Ophthalmology, University of Insubria, Ospedale di Circolo, Via F. Guicciardini 9, 21100 Varese, Italy.
Mediators Inflamm. 2013;2013:857380. doi: 10.1155/2013/857380. Epub 2013 Oct 21.
Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology.
Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control.
Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3.
Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.
增生性玻璃体视网膜病变（PVR）是视网膜脱离的一种严重炎症并发症。病理性视网膜前膜在视网膜表面生长，导致收缩，5%至 10%的病例手术失败。我们评估了 PVR 标本中 VEGF-A、Otx1、Otx2、Otx3 和 p53 家族成员的表达，以关联它们在诱导或预防该疾病中的作用。
在玻璃体视网膜手术中，从接受 PVR 治疗性视网膜切除术的患者中取出 12 个视网膜样本。使用定量实时逆转录聚合酶链反应分析和免疫组织化学，使用 4 个健康人视网膜作为对照，评估基因表达。
对照组显示所有基因的基础表达。PVR 样本中 Otx1 的表达很少或没有，而 VEGF-A、Otx2、Otx3、p53 和 p63 基因的表达则不同。VEGF-A、Otx2、p53 和 p63 之间以及 Otx1 和 Otx3 之间存在显著相关性。
Otx 同源盒、p53 家族和 VEGF-A 基因在 PVR 人视网膜中表达。我们鉴定了两种可能的途径（VEGF-A、Otx2、p53、p63 和 Otx1 和 Otx3）参与 PVR 进展，这些途径可能以不同的方式影响该疾病的进程。鉴定 PVR 的遗传途径是 PVR 治疗的一种新方法。
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