头花蓼序列特异性扩增区域（SCAR）标记的研究
[Study on sequence characterized amplified region (SCAR) markers of Polygonum capitatum].
作者信息
Zhou Tao, Xie Yu, Zhang Li-Yan, Wei Sheng-Hua, Jin Yan-Lei
机构信息
Guiyang college of Traditional Chinese Medicine, Guiyang 550002, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2013 Aug;38(16):2577-80.
OBJECTIVE
To establish sequence characterized amplified region markers of Polygonum capitatum.
METHOD
The random primer was screened through RAPD to obtain the specific RAPD marker band, and the band was separated, extracted, cloned and sequenced. The specific primers were designed for conventional PCR reaction on the basis of the specific band, and the SCAR marker was acquired.
RESULT
Screening from 50 RAPD primer, only C29 primer had 2 specific bands could distinguish P. capitatum from P. nepalense, then 4 pairs of specific primers were designed based on the 2 sequences of RAPD marker bands, and only 1 pair primer (Z1-2) was successfully converted into SCAR marker after repeated tests.
CONCLUSION
The Z1-2 primer, could be used as an effective SCAR mark to identify Z300 DNA for P. capitatum. The SCAR mark was established and can be used as a molecular marker to distinguish P. capitatum from P. nepalense
目的
建立头花蓼的序列特征化扩增区域标记。
方法
通过随机扩增多态性DNA（RAPD）筛选随机引物，获得特异性RAPD标记条带，对该条带进行分离、提取、克隆及测序。基于该特异性条带设计特异性引物用于常规聚合酶链反应（PCR），获得序列特征化扩增区域（SCAR）标记。
结果
从50条RAPD引物中筛选，只有C29引物有2条特异性条带可将头花蓼与尼泊尔蓼区分开，然后基于RAPD标记条带的2条序列设计了4对特异性引物，经反复试验只有1对引物（Z1-2）成功转化为SCAR标记。
结论
Z1-2引物可作为有效的SCAR标记用于鉴定头花蓼的Z300 DNA。已建立SCAR标记，可作为区分头花蓼和尼泊尔蓼的分子标记。
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