Wang Yan-Ming, Wang Ya-Jing, Zhang De-Qin, Du Shou-Ying, Sun Shi-Zhen
Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China.
Zhongguo Zhong Yao Za Zhi. 2013 Aug;38(16):2618-22.
Chonghe gel originated from the Chinese ancient prescription, can be used for the treatment of diabetic foot. This experiment was to study the transdermal absorbability of paeoniflorin and osthole in Chonghe gel . Franz diffusing cells method was adopted for the in vitro model of rat belly skins. Paeoniflorin and osthole in the receiving liquid, skins and gel were determined by HPLC. The receiving liquid were screened, and Chonghe gel and Chonghe ointment were compared by transdermal absorbability. Result showed that ethanol-normal saline (2: 8) solution was the appropriate receiving liquid. The penetration rates of paeoniflorin and osthole were 78.07, 7.08 microg x cm(-2) x h(-1). respectively. In 24 h, the accumulated penetration rates were (31.51 +/- 1.33)%, (12.38 +/- 1.28)%, respectively. The retention rates of paeoniflorin and osthole in skin were (0.92 +/- 0.45)%, (4.81 +/- 1.03) %, respectively. The retention of osthole in skins was a drug reservoir. Transdermal behavior of effective constituents in Chonghe gel was more efficient than that in ointment. In vitro, the transdermal behavior of paeoniflorin in Chonghe gel was close to a Weibull process, while the behavior of osthole was close to Higuchi process.
冲和凝胶源于中国古代方剂，可用于治疗糖尿病足。本实验旨在研究冲和凝胶中芍药苷和蛇床子素的透皮吸收性能。采用Franz扩散池法建立大鼠腹部皮肤体外模型。通过高效液相色谱法测定接收液、皮肤和凝胶中芍药苷和蛇床子素的含量。筛选接收液，并比较冲和凝胶与冲和膏的透皮吸收性能。结果表明，乙醇-生理盐水（2∶8）溶液为适宜的接收液。芍药苷和蛇床子素的渗透速率分别为78.07、7.08μg·cm-2·h-1。24 h内累积渗透率分别为（31.51±1.33）%、（12.38±1.28）%。芍药苷和蛇床子素在皮肤中的滞留率分别为（0.92±0.45）%、（4.81±1.03）%。蛇床子素在皮肤中的滞留可作为药物贮库。冲和凝胶中有效成分的透皮行为比软膏更有效。体外实验中，冲和凝胶中芍药苷的透皮行为接近Weibull过程，而蛇床子素的行为接近Higuchi过程。
