Department of Chemistry and Chemical Biology, McMaster University , 1280 Main Street West, Hamilton, Ontario L8S 4M1, Canada.
Inorg Chem. 2013 Dec 2;52(23):13521-8. doi: 10.1021/ic401972g. Epub 2013 Nov 14.
A convenient method to prepare (99m)Tc analogues of a class of rhenium(I) luminophores was developed, creating isostructural pairs of nuclear and optical probes. A two-step procedure and a new one-pot procedure were used to produce a series of [2 + 1] complexes of the type Tc(CO)3(bipy)L in greater than 80% yield. The plasma stability of the reported compounds was evaluated, where the basicity of the monodentate pyridine type ligand (L) has a significant impact with half-lives ranging from 2 to 20 h. The ability to generate the radioactive complexes makes it possible to quantitate cell uptake of Re luminophores, which was demonstrated in MCF-7 breast cancer cells using (99m)Tc analogues of two Re(I)-based mitochondrial targeting dyes.
开发了一种方便的方法来制备一类铼（I）类发光体的 (99m)Tc 类似物，从而构建了结构相同的核和光学探针对。使用两步法和新的一锅法以大于 80%的产率合成了一系列 [2 + 1] 型 Tc(CO)3(bipy)L配合物。评估了所报道的化合物的血浆稳定性，其中单齿吡啶型配体 (L) 的碱性具有显著影响，半衰期范围为 2 至 20 小时。生成放射性配合物的能力使得定量测定 Re 发光体的细胞摄取成为可能，这在 MCF-7 乳腺癌细胞中使用两种基于 Re(I)的线粒体靶向染料的 (99m)Tc 类似物得到了证明。
