Werner D, Wilcockson J, Zimmermann E
Arch Microbiol. 1975 Sep 30;105(1):27-32. doi: 10.1007/BF00447108.
Ion exchange papers were used to study the adsorption of 32P-labelled rhizobia on defined surfaces. Two strains of Rhizobium japonicum and one each of R. leguminosarum and R. lupini were compared with Escherichia coli and Bacillus subtilis. The ratio of adsorption to strong and to weak acid papers/strong and weak basic papers was consistantly higher for all rhizobial strains compared to the other bacteria. The process of desorption by increasing the ion-concentration causes about 35% desorption between 0.02 and 0.1 M MgCl2, however, an increase to 1 M does not desorb more labelled Rhizobium japonicum or E. coli cells. The ratio of adsorbed cpm to colony formers, desorbed by 0.1 M NaCl was similar with Rhizobium japonicum for all six ion exchange papers. For E. coli this ratio varied widely for the different papers. The selection of Rhizobium against a more closely related bacterium by this adsorption/desorption procedure was demonstrated with mixed cultures of Rhizobium japonicum and Chromobacterium violaceum giving a more than 80 fold enrichment of the former. Rhizobium japonicum cells, ad/desorbed from all ion exchange papers kept their infectivity and formed nodules on Glycine max with an activity of 20-40 nM C2H4-hr(-1)-mg nodule(-1). A desorption of Rhizobium japonicum from soybean roots also occurred by increasing the ion concentration. 2-3 times as many cells were removed in this way compared to washing with water.
离子交换纸被用于研究32P标记的根瘤菌在特定表面的吸附情况。将两株日本根瘤菌、一株豌豆根瘤菌和一株羽扇豆根瘤菌与大肠杆菌和枯草芽孢杆菌进行了比较。与其他细菌相比，所有根瘤菌菌株在强酸纸/强碱纸和弱酸纸/弱碱纸上的吸附比例始终更高。通过增加离子浓度进行解吸的过程在0.02至0.1 M MgCl2之间会导致约35%的解吸，但增加到1 M时，不会使更多标记的日本根瘤菌或大肠杆菌细胞解吸。对于所有六种离子交换纸，0.1 M NaCl解吸出的吸附每分钟计数（cpm）与菌落形成单位的比例在日本根瘤菌中相似。对于大肠杆菌，不同纸张的该比例差异很大。通过这种吸附/解吸程序，在日本根瘤菌和紫色色杆菌的混合培养物中证明了根瘤菌相对于更密切相关细菌的选择，前者富集了80多倍。从所有离子交换纸吸附/解吸的日本根瘤菌细胞保持其感染性，并在大豆上形成根瘤，固氮活性为20 - 40 nM C2H4·hr-1·mg根瘤-1。通过增加离子浓度也会使日本根瘤菌从大豆根上解吸。与用水冲洗相比，以这种方式去除的细胞数量是其2 - 3倍。
