Fachgebiet Zellbiologie und Angewandte Botanik, Fachbereich Biologie der Philipps Universität Marburg, D-35032, Marburg, Germany.
Environ Sci Pollut Res Int. 1998;5(4):202-8. doi: 10.1007/BF02986402.
(14)C-TNT was used to quantify the uptake rate and metabolic turnover of TNT in Phaseolus vulgaris. Seventeen plants were analysed by a special cell fractionation method with polar and nonpolar solvents and enzymes. We obtained three cytoplasmic fractions and five cell wall derived fractions. The recovery rate was 72% as measured by liquid scintillation counting. (14)C partitioned almost in equal amounts with approximately 50% in the cytoplasm and in the cell wall. The majority of the TNT-metabolites are present in the cytoplasm as was shown by GC/ECD and thin layer chromatography. The(14)C in the cell wall is bound probably resulting in long-term immobilisation of these metabolites. We conclude that plants may also be a model for nitroaromatic turnover and immobilisation in soil components.
(14)C-TNT 被用于定量测定菜豆中 TNT 的摄取率和代谢周转率。通过使用极性和非极性溶剂和酶的特殊细胞分级分离方法对 17 株植物进行了分析。我们获得了三个细胞质部分和五个细胞壁衍生部分。通过液体闪烁计数测量，回收率为 72%。(14)C 几乎等量分配，大约 50%在细胞质和细胞壁中。如 GC/ECD 和薄层色谱所示，大多数 TNT 代谢物存在于细胞质中。细胞壁中的(14)C 可能被结合，导致这些代谢物的长期固定。我们得出结论，植物也可能是土壤成分中硝芳烃转化和固定的模型。
