Lassignal N L, Singer J J, Walsh J V
Am J Physiol. 1986 May;250(5 Pt 1):C792-8. doi: 10.1152/ajpcell.1986.250.5.C792.
The contractile effect of various neuropeptides was examined by pressure ejecting these agents from a pipette onto single smooth muscle cells freshly dissociated from the stomach of Bufo marinus. Substance P, cholecystokinin-octapeptide, and bombesin caused contraction, whereas vasoactive intestinal peptide, secretin, and dopamine inhibited acetylcholine-induced contractions. Acetylcholine and the three peptides which produced contraction were found in some instances to act on the same cell, suggesting that receptors for these agents exist on one and the same cell.
通过将各种神经肽从移液管压力喷射到刚从海蟾蜍胃中分离出的单个平滑肌细胞上来检测其收缩效应。P物质、胆囊收缩素八肽和蛙皮素可引起收缩，而血管活性肠肽、促胰液素和多巴胺则抑制乙酰胆碱诱导的收缩。在某些情况下发现乙酰胆碱和三种产生收缩的肽作用于同一细胞，这表明这些物质的受体存在于同一个细胞上。
