Hisada T, Singer J J, Walsh J V
Department of Physiology, University of Massachusetts Medical Center, Worcester 01655.
Pflugers Arch. 1993 Jan;422(4):397-400. doi: 10.1007/BF00374297.
Aluminofluoride (AF) has a variety of biological actions such as activation of GTP binding proteins and inhibition of phosphatases. In the present study, the effects of AF on hyper-polarization- and stretch-activated cationic channels (HA-SACs) were investigated in isolated gastric smooth muscle cells from the toad, Bufo marinus, using the patch-clamp technique. In cell-attached patches extracellular application of AF (20 mM KF plus 20 microM AlCl3) reversibly increased HA-SAC activity without changing its voltage sensitivity. The single channel current amplitude of HA-SACs was not affected during this procedure. The mechanism of AF-induced activation of HA-SACs remains unclear. However, this activation may play a role in contraction of smooth muscle induced by AF.
铝氟化物（AF）具有多种生物学作用，如激活GTP结合蛋白和抑制磷酸酶。在本研究中，采用膜片钳技术，研究了AF对海蟾蜍离体胃平滑肌细胞中超极化和牵张激活阳离子通道（HA-SACs）的影响。在细胞贴附式膜片中，细胞外施加AF（20 mM KF加20 μM AlCl3）可使HA-SAC活性可逆性增加，而不改变其电压敏感性。在此过程中，HA-SACs的单通道电流幅度未受影响。AF诱导HA-SACs激活的机制尚不清楚。然而，这种激活可能在AF诱导的平滑肌收缩中起作用。
