Ben-Aïssa H, Paulie S, Koho H, Biberfeld P, Hansson Y, Braesch-Andersen S, Lagerkvist M, Lundblad M L, Gustafson H, Perlmann P
Anticancer Res. 1986 Mar-Apr;6(2):165-70.
We have previously described the generation of mouse monoclonal antibodies (Mabs) directed to cell surface antigens of human bladder carcinoma. Based on experiments with cultured cells and a limited number of freshly isolated tissues, four distinct antigens were identified as being associated with this disease. In the present investigation, comprising the immunostaining of tissues of normal, malignant, and fetal origin, we have confirmed and extended the close association of these antigens with bladder cancer. Antibodies to all four antigens could clearly discriminate between malignant and normal uroepithelium. Two of the antibodies, SK4H-12 and 4E8, showed no additional reaction when tested with various adult tissues of normal or malignant origin. Antibodies to the remaining two antigens gave a positive staining of a few other tissue types.
我们之前描述了针对人膀胱癌细胞表面抗原的小鼠单克隆抗体（Mabs）的产生。基于对培养细胞和有限数量新鲜分离组织的实验，确定了四种不同的抗原与该疾病相关。在本研究中，包括对正常、恶性和胎儿来源组织的免疫染色，我们证实并扩展了这些抗原与膀胱癌的密切关联。针对所有四种抗原的抗体能够清晰地区分恶性和正常尿路上皮。其中两种抗体，SK4H-12和4E8，在用各种正常或恶性来源的成人组织进行测试时未显示额外反应。针对其余两种抗原的抗体在其他几种组织类型中呈阳性染色。
