Department of Botany, Australian National University, GPO Box 4, 260, Canberra, ACT, Australia.
Theor Appl Genet. 1988 Jul;76(1):54-8. doi: 10.1007/BF00288831.
Provided the nature of inheritance is known, the frequency of homozygous mutant plants in individual M2 families (derived from M1 seed) can be used to estimate the genetically effective cell number (GECN). Segregation ratios in M3 families derived from M2 wild-type plants indicated that the supernodulation characters nts382, nts1007 and nts183 are inherited as Mendelian recessives. The nature of inheritance was also known or confirmed to be recessive by crossing the wild type to these and several other mutants derived from the same population of M2 families. Subsequently, using the frequency of mutant plants in individual M2 families, the GECN for soybean was calculated to be approximately two.
如果知道遗传的性质,那么就可以根据 M2 家系(来自 M1 种子)中纯合突变体植物的频率来估计遗传有效细胞数(GECN)。来自 M2 野生型植物的 M3 家系的分离比表明,nts382、nts1007 和 nts183 这三个超级结瘤特性是孟德尔隐性遗传的。通过将野生型与这些以及来自 M2 家系同一群体的其他几个突变体杂交,也了解或确认了这些突变体的遗传性质是隐性的。随后,使用单个 M2 家系中突变体植物的频率,计算出大豆的 GECN 约为 2。