Department of Botany, Australian National University, Canberra, ACT 2600, Australia.
Plant Physiol. 1985 May;78(1):34-40. doi: 10.1104/pp.78.1.34.
The nodulation characteristics of soybean (Glycine max) mutant nts382 are described. The mutant nodulated significantly more than the parent cultivar Bragg in the presence and absence of several combined nitrogen sources (KNO(3), urea, NH(4)Cl, and NH(4)NO(3)). The number of nodules on the tap root and on lateral roots was increased in the mutant line. In the presence of KNO(3) and urea, nitrogenase activity was considerably higher in nts382 than in Bragg. Mutant plants were generally smaller than wild-type plants. Although nts382 is a supernodulator, inoculation with Rhizobium japonicum was necessary to induce nodule formation and both trial strains CB1809 (= USDA136) and USDA110 elicited the mutant phenotype. Segregation of M(3) progeny derived from a M(2) wild-type plant indicated that the mutant character is inherited as a Mendelian recessive. The mutant is discussed in the context of regulation of nodulation and of hypotheses that have been proposed to explain nitrate inhibition of nodulation.
本文描述了大豆（Glycine max）突变体 nts382 的结瘤特性。在存在和不存在几种混合氮源（KNO3、尿素、NH4Cl 和 NH4NO3）的情况下，突变体的结瘤量明显多于亲本品种 Bragg。在突变系中，主根和侧根上的根瘤数量增加。在存在 KNO3 和尿素的情况下，nts382 的固氮酶活性明显高于 Bragg。突变体植物通常比野生型植物小。尽管 nts382 是一个超级结瘤体，但接种根瘤菌是诱导根瘤形成所必需的，试验菌株 CB1809（=USDA136）和 USDA110 都诱发了突变表型。源自 M2 野生型植物的 M3 后代的分离表明，突变特征是作为孟德尔隐性遗传的。本文讨论了结瘤调控以及解释硝酸盐抑制结瘤的假说。
