Institut für Allgemeine Mikrobiologie, Universität Kiel, Kiel, Germany.
Microb Ecol. 1977 Sep;4(3):189-205. doi: 10.1007/BF02015076.
Two strains ofBacillus sp. and a strain ofBrevibacterium sp., originally isolated from a natural quartzite surface, were characterized and employed as test strains with several methods: acridine orange fluorochromation and epifluorescence microscopy were used for detection of individual cells; scanning and transmission microscopy for studying attachment behavior; replica techniques in combination with electron microscopy for following surface interaction effects; and chemical analysis of SiO2 for detecting possible silica leaching activities. The experimental results clearly showed that the three test strains were able to attach to and grow on the precleaned quartz surfaces. Attachment modes were either by direct sorption mechanisms (Brevibacterium sp. S) or the production of adhesive polymers (Bacillus sp. U andBacillus sp. W). In short-term contact incubation experiments with rich media, neither quartz crystal surface structures nor bacterial cell surfaces appeared to be changed. Likewise, significant biochemical dissolution and mechanical dislocation of SiO2 (which would have indicated rapid bacterial weathering activities) could not be detected. The importance of quartz purity and crystalline structure for the initiation of weathering processes is discussed.
两种芽孢杆菌菌株和一种短杆菌菌株最初是从天然石英表面分离出来的，用几种方法进行了特征描述和测试菌株的应用：吖啶橙荧光染色和荧光显微镜用于检测单个细胞；扫描和透射显微镜用于研究附着行为；复制技术与电子显微镜结合用于跟踪表面相互作用的效果；以及二氧化硅的化学分析用于检测可能的硅溶出活性。实验结果清楚地表明，三种测试菌株能够附着在预清洁的石英表面并生长。附着模式要么是通过直接吸附机制（短杆菌 S 株），要么是产生粘性聚合物（芽孢杆菌 U 株和芽孢杆菌 W 株）。在富营养培养基的短期接触孵育实验中，石英晶体表面结构和细菌细胞表面似乎都没有发生变化。同样，也没有检测到 SiO2 的明显生化溶解和机械位错（这将表明快速的细菌风化活动）。讨论了石英纯度和晶体结构对风化过程启动的重要性。
