Apoptosis. 2014 Mar;19(3):428-35. doi: 10.1007/s10495-013-0941-4.
CIDE domain containing proteins are involved in apoptosis and lipid metabolism, and four CIDE containing proteins, Drep1, Drep2, Drep3, and Drep4, have been identified in fly. In this study, we found that Drep3 interacts with Drep4 via the CIDE domain specifically, which completes the interaction map of Drep system in fly, cyclic interactions: Drep1–Drep2–Drep3–Drep4–Drep1. In addition, we analyzed the dynamic stoichiometry changes of Drep proteins upon binding to their binding partners. Our current studies will help us to understand Drep system in fly as well as CIDE domain for protein–protein interactions.
CIDE 结构域蛋白参与细胞凋亡和脂质代谢，在果蝇中已鉴定出四个含有 CIDE 结构域的蛋白，即 Drep1、Drep2、Drep3 和 Drep4。在本研究中，我们发现 Drep3 通过 CIDE 结构域特异性地与 Drep4 相互作用，从而完成了果蝇 Drep 系统的相互作用图谱，即循环相互作用：Drep1-Drep2-Drep3-Drep4-Drep1。此外，我们分析了 Drep 蛋白与结合伴侣结合时的动态计量变化。我们目前的研究将有助于我们理解果蝇中的 Drep 系统以及 CIDE 结构域的蛋白-蛋白相互作用。
