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本文引用的文献

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Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
In vitro analysis of the complete CIDE domain interactions of the Drep system in fly.在体外分析果蝇 Drep 系统完整 CIDE 结构域相互作用。
Apoptosis. 2014 Mar;19(3):428-35. doi: 10.1007/s10495-013-0941-4.
3
General interaction mode of CIDE:CIDE complex revealed by a mutation study of the Drep2 CIDE domain.通过对 Drep2 CIDE 结构域突变的研究揭示 CIDE:CIDE 复合物的一般相互作用模式。
FEBS Lett. 2013 Apr 2;587(7):854-9. doi: 10.1016/j.febslet.2013.02.031. Epub 2013 Feb 26.
4
Dual apoptotic DNA fragmentation system in the fly: Drep2 is a novel nuclease of which activity is inhibited by Drep3.果蝇中的双重凋亡 DNA 片段化系统：Drep2 是一种新型核酸内切酶，其活性受 Drep3 抑制。
FEBS Lett. 2012 Sep 21;586(19):3085-9. doi: 10.1016/j.febslet.2012.07.056. Epub 2012 Jul 28.
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REFMAC5 for the refinement of macromolecular crystal structures.用于大分子晶体结构精修的REFMAC5
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Phaser crystallographic software.相位结晶学软件。
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Molecular evolution of Cide family proteins: novel domain formation in early vertebrates and the subsequent divergence.Cide家族蛋白的分子进化：早期脊椎动物中新型结构域的形成及随后的分化。
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8
Identification and developmental expression of inhibitor of caspase-activated DNase (ICAD) in Drosophila melanogaster.黑腹果蝇中半胱天冬酶激活的脱氧核糖核酸酶抑制剂（ICAD）的鉴定与发育表达
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Apoptotic DNA fragmentation.凋亡性DNA片段化
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Genes with homology to DFF/CIDEs found in Drosophila melanogaster.在黑腹果蝇中发现的与DFF/CIDEs具有同源性的基因。
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丝状DREP4 CIDE结构域：表征与初步X射线晶体学研究。

Filament-like DREP4 CIDE domain: characterization and preliminary X-ray crystallographic studies.

作者信息

Park Hyun Ho

机构信息

Department of Biochemistry, Yeungnam University, Gyeongsan, Republic of Korea.

出版信息

Acta Crystallogr F Struct Biol Commun. 2017 Aug 1;73(Pt 8):481-485. doi: 10.1107/S2053230X1701069X. Epub 2017 Jul 26.

DOI:10.1107/S2053230X1701069X
PMID:28777092
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5544006/
Abstract

DREP4 is a nuclease from fruit fly that is involved in apoptotic DNA fragmentation. DREP4 contains a conserved CIDE domain that acts as a protein-interaction module and is critical for its function. In this study, it was found that DREP4 CIDE domains form filament-like structures in solution. The length of the highly ordered filament-like structure is dependent on the salt concentration. By adjusting the salt concentration the DREP4 CIDE domain could be crystallized, and X-ray diffraction data were collected to a resolution of 1.9 Å. The crystals were found to belong to the orthorhombic space group P222, with unit-cell parameters a = 53.08, b = 76.58, c = 174.59 Å.

摘要

DREP4是一种来自果蝇的核酸酶，参与凋亡性DNA片段化过程。DREP4含有一个保守的CIDE结构域，该结构域作为蛋白质相互作用模块，对其功能至关重要。在本研究中，发现DREP4 CIDE结构域在溶液中形成丝状结构。高度有序的丝状结构的长度取决于盐浓度。通过调节盐浓度，DREP4 CIDE结构域可以结晶，并收集到分辨率为1.9 Å的X射线衍射数据。发现这些晶体属于正交空间群P222，晶胞参数为a = 53.08，b = 76.58，c = 174.59 Å。