DNA Plant Technology Corporation, 2611 Branch Pike, 08077, Cinnaminson, NJ, USA.
Plant Cell Rep. 1989 Jun;8(6):313-6. doi: 10.1007/BF00716662.
Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures.
玉米（Zea mays L.）原生质体从胚性细胞悬浮液中分离出来，再生植株。供体玉米悬浮培养物是从小孢子诱导的易碎愈伤组织中建立的，来源于商业超甜杂交种（sh2sh2）。与液体薄层法相比，当原生质体在玉米细胞饲养层上培养时，细胞集落的形成频率更高。结果表明，从单倍体细胞培养物中分离的玉米原生质体可以再生出单倍体和二倍体土生植株。
