Laboratory of Soil Microbiology Department of Agronomy, The Pennsylvania State University, 16802, USA.
Microb Ecol. 1976 Mar;3(1):65-73. doi: 10.1007/BF02011454.
AParacoccus sp. which transforms aniline and different halogen-substituted derivatives under aerobic and anaerobic conditions was isolated from the soil. In experiments with(14)C-ring-labeled 4-chloroaniline, approximately 60% of the radioactive material disappeared from the growth medium after incubation under anaerobiosis within 48 hr, but under aerobic conditions no decrease of radioactivity in the growth medium was observed, although 4-chloroaniline was completely metabolized. Acetylation appears to constitute, especially under aerobic conditions, a major transformation mechanism by the bacterium, since almost 50% of the acetylated compound could be detected and identified if aniline, 2-, 3-, and 4-chloroaniline served as substrate. The formation of different metabolites under aerobic and anaerobic conditions clearly indicates the existence of two separate pathways in the metabolism of aniline compounds depending on the oxygen status of the environment.
从土壤中分离到一株能在好氧和厌氧条件下转化苯胺和不同卤素取代衍生物的球形节杆菌（Paracoccus sp.）。在（14）C 环标记的 4-氯苯胺实验中，在厌氧条件下孵育 48 小时后，生长培养基中约 60%的放射性物质消失，但在好氧条件下，生长培养基中的放射性物质没有减少，尽管 4-氯苯胺被完全代谢。乙酰化似乎是细菌的主要转化机制，特别是在好氧条件下，因为如果苯胺、2-、3-和 4-氯苯胺作为底物，几乎可以检测到并鉴定出 50%的乙酰化化合物。在好氧和厌氧条件下形成不同的代谢物，这清楚地表明，取决于环境的氧气状态，苯胺化合物的代谢存在两种独立的途径。
